Kabacoff C M, Carrigan D R
J Virol Methods. 1985 Dec;12(3-4):287-301. doi: 10.1016/0166-0934(85)90140-5.
An enzyme linked immunoassay procedure has been developed which allows accurate quantification of low levels of measles virus antigens in infected central nervous system (CNS) tissues. In its development a systematic study was made of the effects of a large excess of nonviral proteins on the assay results. It was found that controls using single proteins such as bovine serum albumin were inadequate. Further, results indicate that experimental samples and viral antigen standards must be closely matched for their content of CNS tissue proteins since such proteins exert distinctive quantitative effects, both dramatic and subtle.
已开发出一种酶联免疫分析程序，可对感染的中枢神经系统（CNS）组织中低水平的麻疹病毒抗原进行准确定量。在其开发过程中，系统研究了大量非病毒蛋白对检测结果的影响。发现使用单一蛋白质（如牛血清白蛋白）作为对照是不够的。此外，结果表明，实验样品和病毒抗原标准品的中枢神经系统组织蛋白含量必须紧密匹配，因为此类蛋白会产生显著和微妙的独特定量效应。
