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磷酸乙醇胺结合蛋白调控水稻在不同日照长度条件下的抽穗期。

Phosphatidyl Ethanolamine Binding Protein () Regulates the Rice Heading Date under Different Day-Length Conditions.

机构信息

College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang 524088, China.

Plant Protection Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China.

出版信息

Int J Mol Sci. 2024 Jan 24;25(3):1449. doi: 10.3390/ijms25031449.

Abstract

Plant () genes often redundantly duplicate on chromosomes and functionally diverge to modulate reproductive traits. Rice harbors thirteen genes, the functions of which are still not clear, except for the and genes. Here, we identified the molecular detail of in rice reproductive stage. encoding protein contained PEBP domain and localized into the nucleus, which transcripts specifically expressed in the shoot and leaf blade with high abundance. Further GUS-staining results show the promoter activity highly expressed in the leaf and stem. knock-out concurrently exhibited early flowering phenotype under the short- and long-day conditions as compared with wild-type and over-expression plants, which independently regulates flowering without an involved / and / pathway. Further, an AT-hook protein was identified to act as upstream regulator of , as the knock-out elevated the expression by modifying Histone H3 acetylation abundance. According to the dissection of molecular functions, our study expanded the roles intellectual function of in the mediating of a rice heading date.

摘要

植物基因经常在染色体上冗余复制,并在功能上分化,以调节生殖特征。水稻含有 13 个基因,除了 和 基因外,其功能尚不清楚。在这里,我们鉴定了水稻生殖阶段 基因的分子细节。编码蛋白含有 PEBP 结构域,并定位于细胞核中,其转录物特异性地在芽和叶片中高丰度表达。进一步的 GUS 染色结果显示, 启动子在叶片和茎中活性高表达。与野生型和过表达植物相比, 基因敲除在短日和长日条件下均表现出早花表型,它独立于 / 和 / 途径调节开花。此外,鉴定到一个 AT 钩蛋白 作为 的上游调节剂,因为敲除 通过修饰组蛋白 H3 乙酰化丰度来提高 的表达。根据 分子功能的剖析,我们的研究扩展了 在调节水稻抽穗期的作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5325/10855395/a29c5b1b75e9/ijms-25-01449-g001.jpg

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