Sakata H, Tsurudome M, Hishiyama M, Ito Y, Sugiura A
J Virol Methods. 1985 Dec;12(3-4):303-11. doi: 10.1016/0166-0934(85)90141-7.
We have established two enzyme-linked immunosorbent assays (ELISAs) for detection of mumps IgM antibody, i.e., indirect IgM ELISA and IgM capture ELISA, for serodiagnosis of recent mumps infection. In the latter method, peroxidase-conjugated monoclonal antibody to mumps virus was employed. Both methods detected mumps antibody of IgM class only in serum fractions separated by centrifugation through a sucrose density gradient. Optical density values given by both ELISAs were correlated for most sera examined. Indirect IgM ELISA, however, gave a false positive reaction for sera containing both rheumatoid factor and mumps IgG antibody, while giving a false negative reaction for sera containing high titers of mumps IgG antibody. This technique was, therefore, less reliable than IgM capture ELISA. IgM antibody detectable by IgM capture ELISA was present in all patients with mumps by the fifth day of illness and persisted for up to 3 mth in most and up to 5 mth in same cases.
我们建立了两种用于检测腮腺炎IgM抗体的酶联免疫吸附测定法（ELISA），即间接IgM ELISA和IgM捕获ELISA，用于近期腮腺炎感染的血清学诊断。在后一种方法中，使用了与过氧化物酶结合的抗腮腺炎病毒单克隆抗体。两种方法仅在通过蔗糖密度梯度离心分离的血清组分中检测到IgM类的腮腺炎抗体。对于大多数检测的血清，两种ELISA给出的光密度值具有相关性。然而，间接IgM ELISA对同时含有类风湿因子和腮腺炎IgG抗体的血清会产生假阳性反应，而对含有高滴度腮腺炎IgG抗体的血清会产生假阴性反应。因此，该技术不如IgM捕获ELISA可靠。通过IgM捕获ELISA可检测到的IgM抗体在所有腮腺炎患者发病后第5天出现，并在大多数患者中持续长达3个月，在部分患者中持续长达5个月。
