Institute of Digital Agriculture, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China.
Institute of Soil and Water Resources and Environmental Science, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou 310058, China.
Int J Mol Sci. 2024 Jan 25;25(3):1451. doi: 10.3390/ijms25031451.
Light intensity primarily drives plant growth and morphogenesis, whereas the ecological impact of light intensity on the phyllosphere (leaf surface and endosphere) microbiome is poorly understood. In this study, garden lettuce ( L.) plants were grown under low, medium, and high light intensities. High light intensity remarkably induced the leaf contents of soluble proteins and chlorophylls, whereas it reduced the contents of leaf nitrate. In comparison, medium light intensity exhibited the highest contents of soluble sugar, cellulose, and free amino acids. Meanwhile, light intensity resulted in significant changes in the composition of functional genes but not in the taxonomic compositions of the prokaryotic community (bacteria and archaea) in the phyllosphere. Notably, garden lettuce plants under high light intensity treatment harbored more sulfur-cycling and carbon-cycling genes than under low light intensity, both of which were among the 20 most abundant prokaryotic genes in the leaf phyllosphere. Furthermore, the correlations between prokaryotic functional genes and lettuce leaf metabolite groups were examined to disclose their interactions under varying light intensities. The relative abundance of the gene was positively correlated with leaf total chlorophyll content but negatively correlated with leaf nitrate content. In comparison, the relative abundance of the gene was positively correlated with leaf total chlorophyll and carotenoids. Overall, this study revealed that the functional composition of the phyllosphere prokaryotic community and leaf metabolite groups were tightly linked in response to changing light intensities. These findings provided novel insights into the interactions between plants and prokaryotic microbes in indoor farming systems, which will help optimize environmental management in indoor farms and harness beneficial plant-microbe relationships for crop production.
光强主要驱动植物的生长和形态发生，而光强对叶际（叶片表面和内圈）微生物组的生态影响还知之甚少。在本研究中，我们将花园生菜（L.）种植在低、中、高光强下。高光照强度显著诱导叶片可溶性蛋白和叶绿素含量增加，而降低叶片硝酸盐含量。相比之下，中等光照强度表现出最高的可溶性糖、纤维素和游离氨基酸含量。同时，光照强度导致功能基因组成发生显著变化，但对叶际原核生物群落（细菌和古菌）的分类组成没有影响。值得注意的是，与低光照强度相比，高光强处理下的花园生菜植株含有更多的硫循环和碳循环基因，它们都是叶片叶际中最丰富的 20 个原核基因之一。此外，还检查了原核功能基因与生菜叶片代谢物组之间的相关性，以揭示它们在不同光照强度下的相互作用。基因的相对丰度与叶片总叶绿素含量呈正相关，与叶片硝酸盐含量呈负相关。相比之下，基因的相对丰度与叶片总叶绿素和类胡萝卜素呈正相关。总的来说，本研究表明，叶际原核生物群落和叶片代谢物组的功能组成在响应变化的光照强度时紧密相关。这些发现为室内农业系统中植物与原核微生物之间的相互作用提供了新的见解，有助于优化室内农场的环境管理，并利用有益的植物-微生物关系促进作物生产。
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