Yuan Weina, Qin Yongqiang, Zhang Wei, Zhou Wenqian, Feng Guangda, Zhu Honghui, Yao Qing
Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (South China), Ministry of Agriculture and Rural Affairs, Guangdong Province Key Laboratory of Microbial Signals and Disease Control, College of Horticulture, South China Agricultural University, Guangzhou, China.
Key Laboratory of Agricultural Microbiomics and Precision Application (MARA), Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Key Laboratory of Agricultural Microbiome (MARA), State Key Laboratory of Applied Microbiology Southern China, Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou, China.
Front Plant Sci. 2025 Apr 3;16:1532188. doi: 10.3389/fpls.2025.1532188. eCollection 2025.
Phyllosphere microbiome plays important roles in crop adaptation to the changing environments. Perennial woody crops undergo annual cycles with the changing weather parameters and the biological factors, which might shape the phyllosphere microbial community. In this study, we aimed to investigate the dynamics of phyllosphere microbiome of pomelo ( (Burm.) Merr.), an economically important horticultural crops worldwide, and to compare the respective contribution of the weather parameters and the biotic factors to the microbial community assembly, with special focus on the amino acids in leaves. Hi-Seq analysis revealed that both bacterial and fungal communities showed annual cycle dynamics, and the bacterial community in summer was much different from those in other seasons probably due to high temperature and precipitation. However, contribution of the biotic factors (e.g., leaf traits) (12%-29%) to microbial community assembly was higher than that of the weather parameters (4%-15%). Redundancy analysis indicated that the leaf amino acids significantly affected bacterial community while sugars significantly affected fungal community, highlighting the differential patterns of bacterial and fungal community as affected by the biotic factors. Finally, structure equation model showed that the weather parameters influenced microbial community colonizing pomelo leaves both in a direct way and in an indirect way via leaf traits (mainly amino acids). These results demonstrate the primary role of weather parameters and the key role of leaf amino acids in shaping phyllosphere microbiome.
叶际微生物群落在作物适应不断变化的环境中发挥着重要作用。多年生木本作物会随着天气参数和生物因素的变化经历年度周期，这可能会塑造叶际微生物群落。在本研究中，我们旨在调查全球重要经济园艺作物柚子（柚（Burm.）Merr.）叶际微生物群的动态变化，并比较天气参数和生物因素对微生物群落组装的各自贡献，特别关注叶片中的氨基酸。高通量测序分析表明，细菌和真菌群落均呈现年度周期动态，夏季的细菌群落与其他季节的差异很大，这可能是由于高温和降水所致。然而，生物因素（如叶片性状）对微生物群落组装的贡献（12%-29%）高于天气参数（4%-15%）。冗余分析表明，叶片氨基酸显著影响细菌群落，而糖类显著影响真菌群落，突出了生物因素对细菌和真菌群落影响的差异模式。最后，结构方程模型表明，天气参数通过叶片性状（主要是氨基酸）直接和间接影响定殖在柚子叶片上的微生物群落。这些结果证明了天气参数的主要作用以及叶片氨基酸在塑造叶际微生物群中的关键作用。
