is not only involved in the immune process but is also closely related to neurodevelopment according to its molecular evolution. However, what role it plays in neurodevelopment remains unclear. In the present research, we found that, by crossbreeding CXCL14 and CXCL14 mice, the number of CXCL14 mice in their offspring was lower than the Mendelian frequency; CXCL14 mice had significantly fewer neurons in the external pyramidal layer of cortex than CXCL14 mice; and may be involved in synaptic plasticity, neuron projection, and chemical synaptic transmission based on analysis of clinical transcriptome data. The expression of was highest at day 14.5 in the embryonic phase and after birth in the mRNA and protein levels. Therefore, we hypothesized that promotes the development of neurons in the somatic layer of the pyramidal cells of mice cortex on embryonic day 14.5. In order to further explore its mechanism, CXCR4 and CXCR7 were suggested as receptors by Membrane-Anchored Ligand and Receptor Yeast Two-Hybrid technology. Through metabolomic techniques, we inferred that promotes the development of neurons by regulating fatty acid anabolism and glycerophospholipid anabolism.