Department of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Fayoum University, Fayoum, 63514, Egypt.
Department of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Cairo University, Cairo, Egypt.
Hum Genomics. 2024 Feb 12;18(1):18. doi: 10.1186/s40246-024-00578-9.
Infection by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) that causes coronavirus disease 2019 (COVID-19) has a high incidence of spread. On January 30, 2020, the World Health Organization proclaimed a public health emergency of worldwide concern. More than 6.9 million deaths and more than 768 million confirmed cases had been reported worldwide as of June 18, 2023. This study included 51 patients and 50 age- and sex-matched healthy subjects. The present study aimed to identify the expression levels of lncRNA CASC2 and miRNA-21-5p (also known as miRNA-21) in COVID-19 patients and their relation to the clinicopathological characteristics of the disease. The expression levels of noncoding RNAs were measured by RT-PCR technique. Results detected that CASC2 was significantly downregulated while miRNA-21-5p was significantly upregulated in COVID-19 patients compared to healthy subjects. A significant negative correlation was found between CASC2 and miRNA-21-5p. ROC curve analysis used to distinguish COVID-19 patients from controls. MiRNA-21-p serum expression level had a significant positive association with temperature and PO2 (p = 0.04 for each). These findings indicate that CASC2 and miRNA-21-p might be used as potential diagnostic and therapeutic biomarkers in COVID-19.
严重急性呼吸综合征冠状病毒 2（SARS-CoV-2）感染导致 2019 年冠状病毒病（COVID-19）的传播率很高。2020 年 1 月 30 日，世界卫生组织宣布 COVID-19 为全球关注的公共卫生紧急事件。截至 2023 年 6 月 18 日，全球已报告超过 690 万例死亡和超过 7.68 亿例确诊病例。本研究纳入了 51 例 COVID-19 患者和 50 例年龄和性别匹配的健康对照者。本研究旨在确定 COVID-19 患者中长链非编码 RNA（lncRNA）CASC2 和微小 RNA-21-5p（也称为微小 RNA-21）的表达水平及其与疾病临床病理特征的关系。通过 RT-PCR 技术测量非编码 RNA 的表达水平。结果显示，与健康对照者相比，COVID-19 患者中 CASC2 明显下调，而 miRNA-21-5p 明显上调。CASC2 与 miRNA-21-5p 之间存在显著负相关。用于区分 COVID-19 患者和对照者的 ROC 曲线分析。miRNA-21-p 血清表达水平与体温和 PO2 呈显著正相关（p 分别为 0.04）。这些发现表明，CASC2 和 miRNA-21-p 可能作为 COVID-19 的潜在诊断和治疗生物标志物。
