Hernandez Gillian, Hulse-Kemp Amanda M, Storm Amanda R
Department of Biology, Western Carolina University, Cullowhee, NC USA.
Genomics and Bioinformatics Research Unit, USDA-ARS, Raleigh, NC.
MicroPubl Biol. 2024 Jan 28;2024. doi: 10.17912/micropub.biology.000868. eCollection 2024.
A gene of unknown function, Gohir.A02G131900.1, identified in was studied using computational sequence and structure bioinformatic tools. The encoded protein GhGH5BG-A0A1U8NW40 (UniProt A0A1U8NW40) is predicted to be secreted and localized to the cell wall. Homology and conserved residues indicate it belongs to a plant-specific subgroup of the glycoside hydrolase family 5 and likely has exo-1,3-β-glucosidase activity. This subgroup is unique in containing a fascin-like domain which may have evolved a unique glucan binding site of interest for further research.
对在[具体文献中]鉴定出的一个功能未知的基因Gohir.A02G131900.1,使用计算序列和结构生物信息学工具进行了研究。预测编码的蛋白质GhGH5BG-A0A1U8NW40(UniProt A0A1U8NW40)会被分泌并定位于细胞壁。同源性和保守残基表明它属于糖苷水解酶家族5的植物特异性亚组,可能具有外切-1,3-β-葡糖苷酶活性。该亚组独特之处在于含有一个类成束蛋白结构域,其可能进化出了一个独特的葡聚糖结合位点,值得进一步研究。