School of Acupuncture-Moxibustion and Tuina, Beijing University of Chinese Medicine.
School of Acupuncture-Moxibustion and Tuina, Beijing University of Chinese Medicine;
J Vis Exp. 2024 Jan 26(203). doi: 10.3791/65593.
Tuina, as an external treatment method of traditional Chinese medicine, has been proven to have an analgesic effect on peripheral neuropathic pain (pNP) in clinical and basic research. However, the optimal time point for the analgesic effect of tuina may vary according to different injury sensations, affecting the exploration of the initiation mechanism of tuina analgesia. The research used minor chronic constriction injury (minor CCI) model rats to simulate pNP and used the intelligent tuina manipulation simulator to simulate the three methods (point-pressing, plucking, and kneading) and three acupoints (Yinmen BL37, Chengshan BL57, and Yanglingquan GB34) for performing tuina therapy. The study evaluated the changes in pain within 24 h and the optimal time point for the efficacy of tuina analgesia in rats with minor CCI models by testing cold sensitivity threshold (CST), mechanical withdrawal threshold (MWT), and thermal withdrawal latency (TWL). Furthermore, the study evaluated IL-10 and TNF-α expression changes through Elisa detection. The results show that tuina has both immediate and sustained analgesic effects. For the three different injury sensitivity thresholds of CST, MWT, TWL, and two cytokines of IL-10 and TNF-α, the analgesic efficacy of tuina within 24 h after intervention is significantly different at different time points.
推拿作为一种中医外治法，在临床和基础研究中已被证实对周围神经病理性疼痛（pNP）具有镇痛作用。然而，推拿的镇痛效果的最佳时间点可能因不同的损伤感觉而异，这影响了对推拿镇痛起始机制的探索。本研究采用慢性轻度压迫损伤（minor CCI）大鼠模型模拟 pNP，并使用智能推拿操作模拟器模拟三种方法（点按、弹拨和揉捏）和三个穴位（阴陵泉 BL37、承山 BL57 和阳陵泉 GB34）进行推拿治疗。通过测试冷敏感阈值（CST）、机械撤回阈值（MWT）和热撤回潜伏期（TWL），评估了 minor CCI 模型大鼠在 24 小时内疼痛变化和推拿镇痛效果的最佳时间点。此外，通过 Elisa 检测评估了 IL-10 和 TNF-α 表达的变化。结果表明，推拿具有即时和持续的镇痛作用。对于 CST、MWT、TWL 三种不同的损伤敏感阈值以及 IL-10 和 TNF-α 两种细胞因子，干预后 24 小时内推拿的镇痛效果在不同时间点有显著差异。
