Yang Zhenjie, Yu Tianyuan, Chen Jinping, Zhang Runlong, Zhang Yingqi, Liu Jiayue, Zhang Hanyu, Sun Jiawei
School of Acupuncture-Moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing, People's Republic of China.
J Pain Res. 2024 Jan 23;17:321-334. doi: 10.2147/JPR.S438682. eCollection 2024.
This study aimed to investigate changes in metabolomic expression in the spinal dorsal horn (SDH) and thalamus during a Tuina session, aiming to elucidate the mechanism of immediate analgesia.
The rats were randomly divided into three groups: the Sham group, the Model group, and the Tuina group. A minor chronic constriction injury (minor CCI) model was established in both the Model group and the Tuina group. The therapeutic effect of Tuina was determined using the mechanical withdrawal threshold (MWT) and thermal withdrawal latency (TWL) tests. Differential metabolites of the SDH and thalamus were detected using liquid chromatography-tandem mass spectrometry (LC-MS/MS). Bioinformatic analysis was performed using CV, PCA, Venn, and KEGG.
The therapeutic effect of MWT and TWL after instant Tuina intervention was significant. The therapeutic effect of Tuina instant was significantly better compared to the Model group. In the Veen analysis, it was found that Tuina instantly regulates 10 differential metabolites in the SDH and 5 differential metabolites in the thalamus. In the KEGG enrichment analysis, we found that differential metabolites were enriched in 43 pathways in the thalamus and 70 pathways in the SDH.
Tuina therapy may have analgesic effects by metabolizing neurotransmitters such as 2-Picolinic Acid, 5-Hydroxy-Tryptophan Glutathione Betaine-aldehyde-chloride Leucine Lysine Methionine Sarcosine Succinic Acid Histidine Acetylcholine and 5-Hydroxyindoleacetic Acid through the cAMP pathway. It also affects pathways of neurodegeneration-multiple diseases, butanoate metabolism, tyrosine metabolism.
本研究旨在探讨推拿过程中脊髓背角（SDH）和丘脑代谢组学表达的变化，以阐明即时镇痛的机制。
将大鼠随机分为三组：假手术组、模型组和推拿组。模型组和推拿组均建立轻微慢性压迫损伤（轻微CCI）模型。采用机械缩足阈值（MWT）和热缩足潜伏期（TWL）试验测定推拿的治疗效果。使用液相色谱 - 串联质谱（LC-MS/MS）检测SDH和丘脑的差异代谢物。使用CV、PCA、Venn和KEGG进行生物信息学分析。
即时推拿干预后MWT和TWL的治疗效果显著。与模型组相比，即时推拿的治疗效果明显更好。在Veen分析中，发现推拿即时调节SDH中的10种差异代谢物和丘脑中的5种差异代谢物。在KEGG富集分析中，我们发现差异代谢物在丘脑中富集于43条通路，在SDH中富集于70条通路。
推拿疗法可能通过环磷酸腺苷（cAMP）途径代谢2-吡啶甲酸、5-羟基色氨酸、谷胱甘肽甜菜碱 - 醛 - 氯化物、亮氨酸、赖氨酸、蛋氨酸、肌氨酸、琥珀酸、组氨酸、乙酰胆碱和5-羟基吲哚乙酸等神经递质而产生镇痛作用。它还影响神经退行性变 - 多种疾病、丁酸代谢、酪氨酸代谢等通路。
