Amend A, Brückler J, Neubüser U, Blobel H
Zentralbl Bakteriol Mikrobiol Hyg A. 1985 Dec;260(4):443-7. doi: 10.1016/s0176-6724(85)80065-1.
Oligomer 12 S alpha-toxin as well as 3 S alpha-toxoid of Staphylococcus aureus induced the formation of monoclonal antibodies (mabs). Mabs against the 12 S alpha-toxin could be demonstrated in 31 and those against 3 S alpha-toxoid in 18 of 120 hybrid cell colonies. Each of these mab-preparations reacted with 12 S, 3 S alpha-toxin and 3 S alpha-toxoid. The reactions were more pronounced with the homologous than the heterologous toxin preparations. Mabs against 12 S alpha-toxin inhibited the hemolytic effects of native 3 S alpha-toxin as well or better than the respective polyclonal antisera.
