不同化学法去细胞化方案对山羊心包性能的影响。
Impact of the Different Chemical-Based Decellularization Protocols on the Properties of the Caprine Pericardium.
机构信息
Department of Biotechnology and Medical Engineering, National Institute of Technology, Rourkela, Odisha, 769008, India.
出版信息
Cardiovasc Eng Technol. 2024 Jun;15(3):279-289. doi: 10.1007/s13239-024-00712-7. Epub 2024 Feb 12.
PURPOSE
This study aims to decellularized caprine pericardium tissue with varied non-ionic surfactant and anionic detergent concentrations.
METHODS
Protocol A consists of 1%, 0.5%, and 0.25% (w/v) sodium dodecyl sulphate (SDS). Protocol B uses 1%, 0.5%, and 0.25% (w/v) Triton X-100. Protocol C comprised 0.5% SDS + 0.5% Triton X-100, 0.5% + 0.25%, and 0.25% SDS + 0.5% Triton X-100.
RESULTS
Protocol B left a few countable cells in the pericardium tissue, but treatments A and C removed all cells. DNA quantification also demonstrated that protocol B had the most leftover DNA after decellularization. The pericardium tissue treated with an equal combination of anionic detergent and non-ionic surfactant preserves the matrix. However, changing the anionic detergent-non-ionic surfactant ratio disrupted the microstructure. Protocol A decreased pericardium tissue secant modulus (p < 0.05). Protocol B-treated pericardium tissue matched native tissue secant modulus and ultimate tensile stress. Protocol C strengthened pericardium tissue.
CONCLUSION
The intact extracellular matrix and biomechanical properties like native tissues require optimal chemical doses and combinations.
目的
本研究旨在使用不同浓度的非离子表面活性剂和阴离子去污剂对山羊心包组织进行脱细胞处理。
方法
方案 A 包括 1%、0.5%和 0.25%（w/v）的十二烷基硫酸钠（SDS）。方案 B 使用 1%、0.5%和 0.25%（w/v）的 Triton X-100。方案 C 包括 0.5% SDS + 0.5% Triton X-100、0.5% + 0.25%、0.25% SDS + 0.5% Triton X-100。
结果
方案 B 在心包组织中仅留下少数可数细胞，但方案 A 和 C 去除了所有细胞。DNA 定量也表明，脱细胞处理后方案 B 剩余的 DNA 最多。用等量阴离子去污剂和非离子表面活性剂处理的心包膜组织保留了基质。然而，改变阴离子去污剂-非离子表面活性剂的比例会破坏微观结构。方案 A 降低了心包组织的切向模量（p < 0.05）。方案 B 处理的心包膜组织的切向模量和极限拉伸应力与天然组织匹配。方案 C 增强了心包组织。
结论
完整的细胞外基质和类似天然组织的生物力学特性需要最佳的化学剂量和组合。
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