戊二醛 OZAKI 处理人心包膜 10 分钟与 20 分钟对机械性能的影响。
Impact on Mechanical Properties of 10 versus 20 Minute Treatment of Human Pericardium with Glutaraldehyde in OZAKI Procedure.
机构信息
Department of Cardiac Surgery, University Hospital Basel, Basel, Switzerland.
Department of Biomedicine, University of Basel, Basel, Switzerland.
出版信息
Ann Thorac Cardiovasc Surg. 2021 Aug 20;27(4):273-277. doi: 10.5761/atcs.nm.20-00125. Epub 2021 Feb 3.
PURPOSE
The aim of this study was to analyze the effects of 10-minute (standard term) versus 20-minute treatment with glutaraldehyde (GA) on mechanical stability and physical strength of human pericardium in the setting of the OZAKI procedure.
METHODS
Leftover pericardium (6 patients) was bisected directly after the operation, and one-half was further fixed for 10 additional minutes. Uniaxial tensile tests were performed and ultimate tensile strength (UTS), ultimate tensile strain (uts), and collagen elastic modulus were evaluated.
RESULTS
Both treatments resulted in similar values of uniaxial stretching-generated elongations at rupture (10 minutes 25 ± 7 % vs. 20 minutes: 22 ± 5 %; p = 0.05), UTS (5.16 ± 2 MPa vs. 6.54 ± 3 MPa; p = 0.59), and collagen fiber stiffness (elastic modulus: 31.80 ± 15.05 MPa vs. 37.35 ± 15.78 MPa; p = 0.25).
CONCLUSION
Prolongation of the fixation time of autologous pericardium has no significant effect on its mechanical stability; thus, extending the intraoperative treatment cannot be recommended.
目的
本研究旨在分析在 OZAKI 手术中，戊二醛（GA）处理 10 分钟（标准术语）与 20 分钟对人心包膜的机械稳定性和物理强度的影响。
方法
在手术后直接将剩余的心包膜对半切开，其中一半再固定 10 分钟。进行单轴拉伸试验，评估极限拉伸强度（UTS）、极限拉伸应变（uts）和胶原弹性模量。
结果
两种处理方法均导致断裂时的单轴拉伸伸长率相似（10 分钟为 25±7%，20 分钟为 22±5%；p=0.05），UTS（5.16±2 MPa 与 6.54±3 MPa；p=0.59）和胶原纤维硬度（弹性模量：31.80±15.05 MPa 与 37.35±15.78 MPa；p=0.25）。
结论
延长自体心包的固定时间对其机械稳定性没有显著影响；因此，不能推荐延长术中处理时间。
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