Danish Centre for Particle Therapy (DCPT), Aarhus University Hospital, Aarhus Denmark; Department of Experimental Clinical Oncology, Aarhus University Hospital, Aarhus, Denmark; Institute for Biomedicine, Aarhus University, Aarhus, Denmark; Department of Public Health, Department of Biostatistics, Aarhus University, Aarhus, Denmark.
Danish Centre for Particle Therapy (DCPT), Aarhus University Hospital, Aarhus Denmark; Department for Medical Physics, Aarhus University Hospital, Aarhus, Denmark.
Acta Oncol. 2024 Feb 13;63:23-27. doi: 10.2340/1651-226X.2024.19657.
Radiobiological experimental setups are challenged by precise sample positioning along depth dose profile, scattering conditions, and practical difficulties that must be addressed in individual designs. The aim of this study was to produce cell survival curves with several irradiation modalities, by using a setup designed at the Danish Centre for Particle Therapy (DCPT) for in vitro proton irradiations using a horizontal beam line and thereby evaluating the setups use for in vitro irradiations experiments.
The setup is a water phantom suitable for in vitro research with multiple irradiation modalities, in particular the pencil scanning proton beam available from a horizontal experimental beamline. The phantom included a water tank of 39.0 × 17.0 × 20.5 cm. Cell survival-curves were produced using the cell line V79 Chinese hamster lung fibroblast cells (V79s) in biological triplicates of clonogenic assays. Cell survival curves were produced with both a 18 MeV electron beam, 6 MV photon beam, and a Spread-Out Bragg Peak (SOBP) proton beam formed by pristine energies of 85-111 MeV where three positions were examined.
Survival curves with uncertainty areas were made for all modalities. Dosimetric uncertainty amounted to, respectively, 4%, 3% and 3% for proton, electron, and high energy photon irradiations. Cell survival fraction uncertainty was depicted as the standard deviation between replications of the experiment.
Cell survival curves could be produced with acceptable uncertainties using this novel water phantom and cellular laboratory workflow. The setup is useful for future in vitro irradiation experiments.
放射生物学实验装置在精确沿深度剂量分布定位样本、散射条件以及在个别设计中必须解决的实际困难方面受到挑战。本研究的目的是使用丹麦粒子治疗中心（DCPT）设计的水平束线进行体外质子照射的装置，产生具有多种照射方式的细胞存活曲线，并评估该装置在体外照射实验中的用途。
该装置是一种适用于多种照射方式的体外研究的水模体，特别是可从水平实验束线获得的铅笔扫描质子束。水模体包括一个 39.0×17.0×20.5cm 的水箱。使用 V79 中国仓鼠肺成纤维细胞系（V79s）在生物学三重复克隆形成测定中产生细胞存活曲线。使用 18MeV 电子束、6MV 光子束和由 85-111MeV 原始能量形成的扩展布拉格峰（SOBP）质子束产生了存活曲线，其中检查了三个位置。
所有方式都产生了具有不确定区域的存活曲线。质子、电子和高能光子照射的剂量不确定性分别为 4%、3%和 3%。细胞存活分数的不确定性表示为实验重复之间的标准偏差。
使用这种新型水模体和细胞实验室工作流程，可以以可接受的不确定性产生细胞存活曲线。该装置对未来的体外照射实验很有用。
