Department of Microbiology and Immunology, Faculty of Pharmacy, Mansoura University, Mansoura, Egypt.
Department of Endemic Hepatology and Gastroenterology, Faculty of Medicine, Mansoura University, Mansoura, Egypt.
Virus Genes. 2024 Apr;60(2):235-239. doi: 10.1007/s11262-024-02055-w. Epub 2024 Feb 13.
Hepatitis B virus (HBV) vaccine is composed of the purified hepatitis B surface antigen (HBsAg) that is produced by recombinant DNA technology. The neutralizing antibodies induced by vaccination target mainly the "a" determinant, aa124-147, of the outer viral envelope (HBsAg). In the present work, we demonstrate a case study for vaccinated patient that is infected with a vaccine escape HBV strain (Eg200). Characterization of the isolate Eg200 showed that it belongs to the genotype D and an uncommon sub-genotype in Egypt; D9. The DNA sequence encoding HBsAg was sequenced. Mutational analysis of the HBsAg showed a double mutation in the "a" determinant of this HBV isolate; T125M and P127T. However, such substitutions were found to be conserved to the detected serotype, ayw3, of Eg200 isolate. This case report indicates that continuous characterization of breakthrough vaccine escape strains of HBV is essential to develop the immunization strategies against HBV infection.
乙型肝炎病毒（HBV）疫苗由重组 DNA 技术生产的纯化乙型肝炎表面抗原（HBsAg）组成。疫苗诱导的中和抗体主要针对病毒外膜（HBsAg）的“a”决定簇，aa124-147。在本工作中，我们对感染疫苗逃逸 HBV 株（Eg200）的接种患者进行了病例研究。对分离株 Eg200 的特征分析表明，它属于埃及常见的基因型 D 和亚基因型 D9；D9。对 HBsAg 的 DNA 序列进行了测序。HBsAg 的突变分析显示，该 HBV 分离株的“a”决定簇发生了双重突变；T125M 和 P127T。然而，这些取代被发现与 Eg200 分离株的检测到的血清型 ayw3 保守。本病例报告表明，对 HBV 疫苗逃逸株进行连续的特征分析对于开发针对 HBV 感染的免疫策略至关重要。
