Subbiah Abirami, Caswell Drew L, Turner Katerina, Jaiswal Ankit, Avidor-Reiss Tomer
University of Toledo, Toledo, Ohio, United States.
MicroPubl Biol. 2024 Jan 29;2024. doi: 10.17912/micropub.biology.001083. eCollection 2024.
Centrioles form centrosomes that organize microtubules, assist in cell structure, and nucleate cilia that provide motility and sensation. Within the sperm, the centrosome consists of two centrioles (proximal and distal centriole) and a pericentriolar material known as the striated column and capitulum. The distal centriole nucleates the flagellum. Mice spermatozoa, unlike other mammal spermatozoa (e.g., human and bovine), have no ultra-structurally recognizable centrioles, but their neck has the centriolar proteins POC1B and FAM161A, suggesting mice spermatozoa have remnant centrioles. Here, we examine whether other centriolar proteins, CP110 and CEP135, found in the human and bovine spermatozoa centrioles are also found in the mouse spermatozoa neck. CP110 is a tip protein controlling ciliogenesis, and CEP135 is a centriole-specific structural protein in the centriole base of canonical centrioles found in most cell types. Here, we report that CP110 and CEP135 were both located in the mice spermatozoa neck around the proximal centriolar remnants labeled by POC1B, increasing the number of centriolar proteins found in the mice spermatozoa neck, further supporting the hypothesis that a remnant proximal centriole is present in mice.
中心粒形成中心体，中心体组织微管、辅助细胞结构并使纤毛成核，纤毛提供运动性和感觉功能。在精子中，中心体由两个中心粒（近端和远端中心粒）和一种称为横纹柱和小头的中心粒周围物质组成。远端中心粒使鞭毛成核。与其他哺乳动物精子（如人类和牛的精子）不同，小鼠精子在超微结构上没有可识别的中心粒，但其颈部含有中心粒蛋白POC1B和FAM161A，这表明小鼠精子有残余中心粒。在此，我们研究人类和牛精子中心粒中发现的其他中心粒蛋白CP110和CEP135是否也存在于小鼠精子颈部。CP110是一种控制纤毛发生的顶端蛋白，而CEP135是在大多数细胞类型中发现的典型中心粒中心粒基部的一种中心粒特异性结构蛋白。在此，我们报告CP110和CEP135都位于小鼠精子颈部围绕由POC1B标记的近端中心粒残余物的位置，增加了在小鼠精子颈部发现的中心粒蛋白的数量，进一步支持了小鼠存在残余近端中心粒的假说。
