Turner Katerina A, Kluczynski Derek F, Hefner Ryan J, Moussa Rami B, Slogar Julia N, Thekkethottiyil Joice B, Prine Haley D, Crossley Emily R, Flanagan Lucas J, LaBoy Marlena M, Moran Mia B, Boyd Taylor G, Kujawski Benjamin A, Ruble Kelsie, Pap John M, Jaiswal Ankit, Shah Tariq A, Sindhwani Puneet, Avidor-Reiss Tomer
The University of Toledo, Toledo, Ohio, USA.
MicroPubl Biol. 2022 Nov 12;2022. doi: 10.17912/micropub.biology.000678. eCollection 2022.
Sperm cells are transcriptionally and translationally silent. Therefore, they may use one of the remaining mechanisms to respond to stimuli in their environment, the post-translational modification of their proteins. Here we examined three post-translational modifications, acetylation, glutamylation, and glycylation of the protein tubulin in human and cattle sperm. Tubulin is the monomer that makes up microtubules, and microtubules constitute the core component of both the sperm centrioles and the axoneme. We found that the sperm of both species were labeled by antibodies against acetylated tubulin and glutamylated tubulin.
精子细胞在转录和翻译水平上处于沉默状态。因此，它们可能会利用剩余的机制之一来响应周围环境中的刺激，即对其蛋白质进行翻译后修饰。在这里，我们研究了人类和牛精子中微管蛋白的三种翻译后修饰，即乙酰化、谷氨酰胺化和糖基化。微管蛋白是构成微管的单体，而微管是精子中心粒和轴丝的核心组成部分。我们发现，这两个物种的精子都被抗乙酰化微管蛋白和抗谷氨酰胺化微管蛋白的抗体标记。
