Ordóñez Yadira F, Miranda Estefanía, López María Fernanda, Ordóñez Paola E
Grupo de Investigación Productos Naturales Bioactivos, Escuela de Ciencias Agrícolas y Ambientales, Pontificia Universidad Católica del Ecuador-Ibarra, Av. Jorge Guzmán Rueda y Av. Padre Aurelio Espinosa Polit, 100112, Ibarra, Ecuador.
Escuela de Ciencias Agrícolas y Ambientales, Pontificia Universidad Católica del Ecuador-Ibarra, Av. Jorge Guzmán Rueda y Av. Padre Aurelio Espinosa Polit, 100112, Ibarra, Ecuador.
Heliyon. 2024 Feb 2;10(3):e25226. doi: 10.1016/j.heliyon.2024.e25226. eCollection 2024 Feb 15.
Lymphadenitis is a commonly occurring and contagious disease in guinea pigs caused by different pathogens, including sp., sp., and sp. This study aimed to characterize the bacteria isolated from pus extracted from abscessed mandibular lymph nodes of diseased guinea pigs in Ecuador in 2019 and evaluate the in vitro antibacterial activity of the total extracts of three plant species. Isolates were recovered from three diseased guinea pigs with Lymphadenitis on a farm in Imbabura, Ecuador province. The bacteria were characterized through microbiological, biochemical, and molecular tests as subsp. . Furthermore, the susceptibility of subsp. to three plant extracts belonging to the Asteraceae family, collected in Ecuador, were assessed in vitro by the microdilution method. Our data indicate that all the evaluated extracts showed activity, with a Minimum Inhibitory Concentration (MIC) of 22.50 mg/mL for , 11.25 mg/mL for , and 5.60 mg/mL for . extract showed the highest efficacy with a % inhibition of 63.90 at the highest tested concentration (45 mg/mL). This is the first report on the bioactivity of these plant species against subsp. .
淋巴结炎是豚鼠中常见的传染性疾病,由不同病原体引起,包括 种、 种和 种。本研究旨在鉴定2019年从厄瓜多尔患病豚鼠脓肿下颌淋巴结中提取的脓液中分离出的细菌,并评估三种植物物种总提取物的体外抗菌活性。分离菌株取自厄瓜多尔因巴布拉省一个农场的三只患有淋巴结炎的患病豚鼠。通过微生物学、生化和分子测试将该细菌鉴定为 亚种。此外,采用微量稀释法在体外评估了厄瓜多尔采集的菊科三种植物提取物对 亚种的敏感性。我们的数据表明,所有评估的提取物均显示出活性, 提取物的最低抑菌浓度(MIC)为22.50 mg/mL, 提取物为11.25 mg/mL, 提取物为5.60 mg/mL。 提取物在最高测试浓度(45 mg/mL)下显示出最高疗效,抑菌率为63.90%。这是关于这些植物物种对 亚种生物活性的首次报道。