School of Chemistry and Chemical Engineering, Center of Free Electron Laser & High Magnetic Field, Key Laboratory of Structure and Functional Regulation of Hybrid Materials Ministry of Education, Key Laboratory of Functional Inorganic Materials Chemistry of Anhui Province, and Key Laboratory of Chemistry for Inorganic/Organic Hybrid Functionalized Materials of Anhui Province, Anhui University, Hefei 230601, P. R. China.
School of Chemical and Environmental Engineering, Anhui Polytechnic University, Wuhu 241000, P. R. China.
Anal Chem. 2024 Feb 27;96(8):3535-3543. doi: 10.1021/acs.analchem.3c05390. Epub 2024 Feb 14.
Currently, monitoring of the adenosine triphosphate (ATP) level in lysosomes is critical to understand their involvement in various biological processes, but it remains difficult due to the interferences of limited targeting and low resolution of fluorescent probes. Herein, we report a classic Mn(II) probe () with near-infrared (NIR) nonlinear (NLO) properties, accompanied by three-four photon transition and fivefold fluorescence enhancement in the presence of ATP. combines with ATP through dual recognition sites of diethoxy and manganese ions to reflect slightly fluorescence lifetime change. Through the synergy of multiphoton fluorescence imaging (MP-FI) and multiphoton fluorescence lifetime imaging microscopy (MP-FLIM), it is further demonstrated that displays lysosome-specific targeting behavior, which can monitor lysosome-related ATP migration under NIR laser light. This work provides a novel multiphoton transformation fluorescence complex, which might be a potential candidate as a simple and straightforward biomarker of lysosome ATP for clinical diagnosis.
目前，监测溶酶体中的三磷酸腺苷 (ATP) 水平对于了解它们在各种生物过程中的作用至关重要，但由于荧光探针的靶向性有限和分辨率低，这仍然很困难。在此，我们报告了一种具有近红外（NIR）非线性（NLO）性质的经典 Mn(II) 探针 ()，在存在 ATP 的情况下，它伴随着三到四光子跃迁和五倍荧光增强。 通过二乙氧基和锰离子的双重识别位点与 ATP 结合，以反映出荧光寿命的轻微变化。通过多光子荧光成像（MP-FI）和多光子荧光寿命成像显微镜（MP-FLIM）的协同作用，进一步证明 具有溶酶体特异性靶向行为，可在近红外激光下监测与溶酶体相关的 ATP 迁移。这项工作提供了一种新型的多光子转化荧光配合物，它可能是一种潜在的候选物，作为临床诊断中溶酶体 ATP 的简单而直接的生物标志物。
