Inner Mongolia Key Laboratory of Toxicant Monitoring and Toxicology, College of Animal Science and Technology, Inner Mongolia Minzu University, Tongliao, Inner Mongolia, 028000, China.
State Key Laboratory of Toxicology and Medical Countermeasures, Institute of Toxicology and Pharmacology, Academy of Military Medical Sciences, Beijing, 100850, China.
Bull Environ Contam Toxicol. 2024 Feb 14;112(2):39. doi: 10.1007/s00128-024-03867-0.
Acetaminophen (N-acetyl-p-aminophenol; APAP) is one of the most widely used analgesics. To examine the toxicity of APAP, we used zebrafish embryos as model animals to detect the effect of APAP on the thyroid system of zebrafish embryos. The zebrafish embryos were exposed to APAP from 4 h post fertilization (4 hpf) until observation. The experimental results showed that APAP caused pericardial edema and decreased pigmentation in the zebrafish embryos or larvae. The APAP treatment caused a decrease in the expression of tpo and thrβ in the zebrafish at 36 and 72 hpf. The transcriptomic analysis found that APAP affected retinol metabolism, the metabolism of xenobiotics by cytochrome P450, and the tyrosine metabolism pathway. The harmful effect of APAP on zebrafish embryos might be due to its disrupting effect on the functional regulation of the thyroid hormone system.
对乙酰氨基酚（N-乙酰对氨基酚；APAP）是最广泛使用的镇痛药之一。为了研究 APAP 的毒性，我们使用斑马鱼胚胎作为模型动物，检测 APAP 对斑马鱼胚胎甲状腺系统的影响。斑马鱼胚胎从受精后 4 小时（4 hpf）开始暴露于 APAP 中，直到观察结束。实验结果表明，APAP 导致斑马鱼胚胎或幼鱼的心包水肿和色素沉着减少。APAP 处理导致 36 和 72 hpf 时斑马鱼中 tpo 和 thrβ 的表达减少。转录组分析发现，APAP 影响视黄醇代谢、细胞色素 P450 介导的外源物代谢以及酪氨酸代谢途径。APAP 对斑马鱼胚胎的有害影响可能是由于其对甲状腺激素系统功能调节的破坏作用。
