Department of Biomaterials and Composites, Faculty of Materials Science and Ceramics, AGH University of Krakow, 30-059 Krakow, Poland.
Department of Ceramics and Refractories, Faculty of Materials Science and Ceramics, AGH University of Krakow, 30-059 Krakow, Poland.
J Mech Behav Biomed Mater. 2024 Apr;152:106437. doi: 10.1016/j.jmbbm.2024.106437. Epub 2024 Feb 3.
The multiscale approach in designing substrates for regenerative medicine endows them with beneficial properties determining their performance in the body. Substrates for corneal regeneration should reveal the proper transparency, mechanical properties and microstructure to maintain the functionality of the regenerated tissue. In our study, series of non-wovens with different fibres orientation (random (R), aligned (A)), topography (shish-kebab (KK), core-shell (CS)) and thickness were fabricated via electrospinning. The samples were assessed for mechanical (static tensile test) and optical properties (spectroscopy UV-Vis). The research evaluated the impact of different microstructures on the viability and morphology of three cell lines (Hs 680, HaCaT and RAW 264.7). The results showed how the fibres arrangement influenced mechanical behaviour of the non-wovens. The randomly oriented fibres were more elongated (up to 50 mm) and had a lower maximum tensile force (up to 0.46 N). In turn, the aligned fibres were characterized by lower elongation (up to 19 mm) and higher force (up to 1.45 N). The conducted transparency tests showed the relation between thickness (of the non-woven and fibres) and morphology of the substrate and light transmission. To simulate the in vivo conditions, prior to the light transmission studies, samples were immersed in water. All the samples exhibited high transparency after immersion in water (>80%). The impact of various morphologies was observed in the in vitro studies. All the samples proved high cells viability. Moreover, the substrate morphology had a significant impact on the orientation and arrangement of the fibroblast cytoskeleton. The aligned fibres were oriented in exactly the same direction. The conducted research proved that, by altering the non-wovens microstructure, the properties can be adjusted so as to induce the desirable cellular reaction. This indicates the high potential of electrospun fibres in terms of modulating the corneal cell behaviour in response to the implanted substrate.
在设计再生医学基底时采用多尺度方法，使它们具有决定其在体内性能的有益特性。用于角膜再生的基底应具有适当的透明度、机械性能和微观结构，以维持再生组织的功能。在我们的研究中，通过静电纺丝制备了具有不同纤维取向（随机（R）、定向（A））、形貌（串珠（KK）、核壳（CS））和厚度的一系列非织造布。对样品进行了机械性能（静态拉伸试验）和光学性能（光谱 UV-Vis）评估。该研究评估了不同微观结构对三种细胞系（Hs 680、HaCaT 和 RAW 264.7）活力和形态的影响。结果表明纤维排列如何影响非织造布的机械性能。随机取向的纤维更长（长达 50 毫米），最大拉伸力更小（高达 0.46 牛）。相反，定向纤维的伸长率较低（长达 19 毫米），力较高（高达 1.45 牛）。进行的透明度测试表明，非织造布和纤维的厚度与基底的形态和光传输之间存在关系。为了模拟体内条件，在进行透光率研究之前，将样品浸入水中。所有样品在浸入水中后（>80%）均表现出高透明度。在体外研究中观察到各种形态的影响。所有样品均证明具有高细胞活力。此外，基底形态对成纤维细胞细胞骨架的取向和排列有显著影响。定向纤维完全定向在同一方向上。进行的研究证明，通过改变非织造布的微观结构，可以调整其性能，从而诱导所需的细胞反应。这表明静电纺丝纤维在调节角膜细胞对植入基底的反应方面具有很高的潜力。
