Peng Bo, He Xueyan, Wang Chunjuan, Du Fengxiang, Zhou Min, Zhao Shengguo, Fang Yanjun
Key Laboratory of Eco-functional Polymer Materials of the Ministry of Education, Key Laboratory of Bioelectrochemistry & Environmental Analysis of Gansu Province, College of Chemistry and Chemical Engineering, Northwest Normal University, Lanzhou 730070, PR China.
Key Laboratory of Eco-functional Polymer Materials of the Ministry of Education, Key Laboratory of Bioelectrochemistry & Environmental Analysis of Gansu Province, College of Chemistry and Chemical Engineering, Northwest Normal University, Lanzhou 730070, PR China.
Food Chem. 2024 Jun 30;444:138624. doi: 10.1016/j.foodchem.2024.138624. Epub 2024 Feb 3.
A novel ratiometric fluorescent probe based on non-conjugated polymer dots (NCPDs) and gold nanocluster (AuNCs) was constructed to determine tert-butylhydroquinone (TBHQ). The probe exhibited dual emission peaks at 480 nm and 630 nm under 370 nm excitation. The fluorescence of AuNCs was quenched by TBHQ due to strong electrostatic interactions, whereas the emission of NCPDs increased. The ratio of fluorescence intensity at 480 nm to 630 nm (F / F) was monitored as analytical signal response. The probe have been utilized for the detection of TBHQ with good linear relationship in the range of 0.2 to 60 μg/mL. The limit of detection (LOD) and the limit of quantitation (LOQ) were 0.048 μg/mL and 0.159 μg/L, respectively. Three levels of spiked-in TBHQ concentrations were obtained with recovery rates from 80 % to 102 %. The present study provided an effective ratiometric fluorescence method for selective screening of TBHQ in food samples.
构建了一种基于非共轭聚合物点（NCPDs）和金纳米簇（AuNCs）的新型比率荧光探针，用于测定特丁基对苯二酚（TBHQ）。该探针在370 nm激发下，在480 nm和630 nm处呈现双发射峰。由于强静电相互作用，TBHQ使AuNCs的荧光猝灭，而NCPDs的发射增强。监测480 nm与630 nm处荧光强度之比（F / F）作为分析信号响应。该探针已用于检测TBHQ，在0.2至60μg/mL范围内具有良好的线性关系。检测限（LOD）和定量限（LOQ）分别为0.048μg/mL和0.159μg/L。获得了三个加标水平的TBHQ浓度，回收率为80%至102%。本研究为食品样品中TBHQ的选择性筛选提供了一种有效的比率荧光方法。
