Yao Lihong, Xu Wanqiu, Liu Lixue, Xu Xiaohang, Xi Hualei, Xue Bing, Cao Xiaofang, Lin Song, Piao Guiyan, Sun Jian, Wang Xiumei
Department of Stomatology, The Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang Province, 150001, China.
Mudanjiang Medical University, Mudanjiang, Heilongjiang Province, China.
BMC Oral Health. 2024 Feb 14;24(1):238. doi: 10.1186/s12903-024-03953-z.
Facial nerve injury often results in poor prognosis due to the challenging process of nerve regeneration. Neuregulin-1, a human calmodulin, is under investigation in this study for its impact on the reparative capabilities of Dental Pulp Stem Cells (DPSCs) in facial nerve injury.
Lentivirus was used to transfect and construct Neuregulin-1 overexpressed DPSCs. Various techniques assessed the effects of Neuregulin-1: osteogenic induction, lipid induction, Reverse Transcription Polymerase Chain Reaction, Western Blot, Cell Counting Kit-8 assay, wound healing, immunofluorescence, Phalloidin staining, nerve stem action potential, Hematoxylin-eosin staining, transmission electron microscopy, and immunohistochemistry.
Neuregulin-1 effectively enhanced the proliferation, migration, and cytoskeletal rearrangement of DPSCs, while simultaneously suppressing the expression of Ras homolog gene family member A (RhoA) and Microfilament actin (F-actin). These changes facilitated the neural differentiation of DPSCs. Additionally, in vivo experiments showed that Neuregulin-1 expedited the restoration of action potential in the facial nerve trunk, increased the thickness of the myelin sheath, and stimulated axon regeneration.
Neuregulin-1 has the capability to facilitate the repair of facial nerve injuries by promoting the regenerative capacity of DPSCs. Thus, Neuregulin-1 is a significant potential gene in the reparative processes of nerve damage.
由于神经再生过程具有挑战性，面神经损伤往往导致预后不良。本研究正在探究神经调节蛋白-1（一种人钙调蛋白）对牙髓干细胞（DPSCs）在面神经损伤中的修复能力的影响。
使用慢病毒转染并构建过表达神经调节蛋白-1的DPSCs。采用多种技术评估神经调节蛋白-1的作用：成骨诱导、脂质诱导、逆转录聚合酶链反应、蛋白质免疫印迹法、细胞计数试剂盒-8检测、伤口愈合实验、免疫荧光、鬼笔环肽染色、神经干动作电位检测、苏木精-伊红染色、透射电子显微镜检查和免疫组织化学。
神经调节蛋白-1有效增强了DPSCs的增殖、迁移和细胞骨架重排，同时抑制了Ras同源基因家族成员A（RhoA）和微丝肌动蛋白（F-肌动蛋白）的表达。这些变化促进了DPSCs的神经分化。此外，体内实验表明，神经调节蛋白-1加速了面神经干动作电位的恢复，增加了髓鞘厚度，并刺激了轴突再生。
神经调节蛋白-1具有通过促进DPSCs的再生能力来促进面神经损伤修复的能力。因此，神经调节蛋白-1是神经损伤修复过程中的一个重要潜在基因。
