College of Pharmaceutical Engineering of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, China.
State Key Laboratory of Component-based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, China.
Rapid Commun Mass Spectrom. 2024 Mar 30;38(6):e9693. doi: 10.1002/rcm.9693.
The volatile organic compounds (VOCs) of Lonicerae Japonicae flos (LJF) and Lonicera flos (LF) play a pivotal role in determining their sensory characteristics, medicinal properties, and subsequent impact on market pricing and consumer preferences. However, the differences and specificity of these VOCs remain obscure. Hence, it is crucial to conduct a comprehensive characterization of the VOCs in LJF and LF and pinpoint their potential differential VOCs.
In this study, headspace gas chromatography-ion mobility spectrometry (HS-GC/IMS) and headspace solid-phase microextraction gas chromatography-mass spectrometry (HS-SPME-GC/MS) were employed to comprehensively investigate the compositional characteristics and distinctions in VOCs between LJF and LF. Multivariate statistical analysis was used to identify candidate differential VOCs of LJF and LF samples.
A total of 54 and 88 VOCs were identified using HS-GC/IMS and HS-SPME-GC/MS analysis, respectively. Primary VOCs detected in LJF include leaf alcohol, (E)-2-hexen-1-ol dimer, 2-octyn-1-ol, and (E)-3-hexen-1-ol. Key VOCs prevalent in LF encompass farnesol, heptanoic acid, octanoic acid, and valeric acid. Multivariate statistical analysis indicates that compounds such as phenethyl alcohol and leaf alcohol were selected as potential VOCs for distinguishing between LJF and LF.
This research conducted a comprehensive analysis of the fundamental volatile components in both LJF and LF. It subsequently elucidated the distinctions and specificities within their respective VOC profiles. And this study enables differentiation between LJF and LF through the analysis of VOCs, offering valuable insights for enhancing the quality control of both LJF and LF.
忍冬花（LJF）和金银花（LF）的挥发性有机化合物（VOCs）在决定其感官特征、药用特性以及对市场定价和消费者偏好的后续影响方面起着关键作用。然而，这些 VOC 的差异和特异性仍然不清楚。因此，对 LJF 和 LF 中的 VOC 进行全面表征并确定其潜在的差异 VOC 是至关重要的。
本研究采用顶空气相色谱-离子迁移谱（HS-GC/IMS）和顶空固相微萃取气相色谱-质谱联用（HS-SPME-GC/MS）技术，全面研究了 LJF 和 LF 中 VOC 的组成特征和差异。采用多元统计分析方法鉴定 LJF 和 LF 样品的候选差异 VOC。
采用 HS-GC/IMS 和 HS-SPME-GC/MS 分析分别鉴定出 54 种和 88 种 VOC。LJF 中检测到的主要 VOC 包括叶醇、（E）-2-己烯-1-醇二聚体、2-辛炔-1-醇和（E）-3-己烯-1-醇。LF 中主要的 VOC 包括法呢醇、庚酸、辛酸和缬草酸。多元统计分析表明，苯乙醇和叶醇等化合物被选为区分 LJF 和 LF 的潜在 VOC。
本研究对 LJF 和 LF 的基本挥发性成分进行了全面分析，阐明了它们各自 VOC 谱中的差异和特异性。通过对 VOC 的分析可以区分 LJF 和 LF，为提高 LJF 和 LF 的质量控制提供了有价值的见解。
