Zhou Lei, Liu Danlei, Zhu Yongqiang, Zhang Zilong, Chen Shiwen, Zhao Guoping, Zheng Huajun
Department of Microbiology and Immunology, School of Life Sciences, Fudan University, Shanghai 200438, People's Republic of China.
Shanghai-MOST Key Laboratory of Health and Disease Genomics, Shanghai Institute for Biomedical and Pharmaceutical Technologies (SIBPT), Fudan University, Shanghai 200032, People's Republic of China.
Heliyon. 2024 Feb 5;10(3):e25642. doi: 10.1016/j.heliyon.2024.e25642. eCollection 2024 Feb 15.
is a significant cause of foodborne illness, and its incidence worldwide is on the rise. It is thus imperative to develop a straightforward and efficient method for typing strains of this pathogen. In this study, we conducted a pangenome analysis of 75 complete genomes of and identified the core gene with the highest degree of variation, which distinguished 44 strains and outperformed traditional seven-gene-based MLST when combined with , another core gene with high degree of variation. The gene had higher resolution to type strains with a close relationship compared to the traditional MLST genes in the phylogenetic tree built by core genomes. Strong positive selection was also detected in the gene (ω > 1), representing adaptive and evolution in response to the environment. Therefore, the panel of gene and may serve as a tool for the typing of , potentially contributing to the prevention and control of this foodborne disease.
是食源性疾病的一个重要病因，其在全球的发病率呈上升趋势。因此，开发一种简单有效的该病原体菌株分型方法势在必行。在本研究中，我们对75个[病原体名称]的完整基因组进行了泛基因组分析，鉴定出变异程度最高的核心基因[基因名称1]，该基因可区分44个菌株，并且与另一个变异程度高的核心基因[基因名称2]结合时，优于传统的基于七个基因的多位点序列分型（MLST）。在由核心基因组构建的系统发育树中，与传统的MLST基因相比，[基因名称1]基因对亲缘关系较近的菌株分型具有更高的分辨率。在基因[基因名称1]中还检测到强烈的正选择（ω > 1），代表对环境的适应性和进化。因此，[基因名称1]和[基因名称2]基因组合可作为[病原体名称]分型的工具，可能有助于预防和控制这种食源性疾病。
