a novel typing gene offering enhanced resolution for pandemic species.

The gene , encoding the mannitol transporter subunit IICBA of the phosphotransferase system, was the core gene with the greatest variability in and could be used as a new typing marker in . To expand its application, we performed an evolutionary analysis and found that the gene was present in nine phyla, 371 genera, and 1,662 species of bacteria. It is commonly found in pathogenic species of , followed by , , etc. To investigate whether the gene could serve as a novel typing gene for whole species, we compared the typing effect of the gene with multilocus sequence type (MLST) in 13 common pathogenic species. The results revealed that the gene was present in all common pathogenic species, providing comparable or even higher typing resolution than MLST, especially in , , , and , enabling accurate typing and traceability; however, many species lacked allelic profiles of MLST genes, making it impossible to obtain accurate STs. In summary, these findings validated the potential typing application of the gene in species.IMPORTANCE is a collection of gram-negative bacteria characterized by possessing two chromosomes, which exhibit numerous shared biological and genomic traits. Around 13 species of are known to cause infections in humans. Without appropriate medical interventions, the consequences of these infections can escalate to become life-threatening. Therefore, based on the prevalent characteristics of pathogenic , it is imperative for us to classify and trace various species that cause human illness and prescribe medication rationally to control the spread of diseases. In our investigation, we explored the prevalence and typing accuracy of the gene in . Our findings revealed its ubiquitous presence across typical pathogenic species, offering superior typing efficacy compared with MLST and more extensive implications.