[放射敏感性基因照射及基因型对酵母细胞减数分裂的影响]
[Effect of radiosensitivity gene irradiation and genotype on yeast cytoduction].
作者信息
Stepanova V P, Zakharov I A
出版信息
Genetika. 1979;15(7):1177-85.
Effects of gamma- and UV-irradiation of one parent on progenies of yeast crosses are studied. In the crosses of the type: p+ a alc4 X p-alpha ade2 (alc4--nuclear respiration deficiency) zygotic colonies (white) and haploid p+ cytoductant colonies (red) were scored. The irradiation of p+ parent increases the cytoductant frequency up to 17%. When both parents are radiation-sensitive (xrs1, allelic to rad54), the frequency of cytoduction reaches 90%. Crosses p+xrs1 X p-xrs+ or p+xrs+ X p-xrs1 are similar to crosses of wild types. The same results were observed in the case of xrs4 mutants as well as for UV-irradiation. In the progenies of crosses irradiated p+ a alc4 (xrs+ or xrs1) X non-irradiated p-alpha ade2 ura (xrs+ or xrs1) the genotype of red colonies was analysed: in most cases (82.3%-99.8%) they were p+ alpha ade2 ura, that was true haploid cytoductants. It is concluded that irradiation induces particular damages of yeast nuclei leading to a block of normal karyogamy and thus to a cytoductant formation. The highest frequency of cytoduction was observed in crosses of radiosensitive mutants.
研究了对酵母杂交后代中一个亲本进行γ射线和紫外线照射的影响。在以下类型的杂交中：p⁺ a alc4×p⁻α ade2（alc4——核呼吸缺陷），对合子菌落（白色）和单倍体p⁺细胞融合菌落（红色）进行了计数。对p⁺亲本的照射使细胞融合频率提高到17%。当双亲均为辐射敏感型（xrs1，与rad54等位）时，细胞融合频率达到90%。p⁺xrs1×p⁻xrs⁺或p⁺xrs⁺×p⁻xrs1的杂交与野生型杂交相似。在xrs4突变体以及紫外线照射的情况下也观察到了相同的结果。在经照射的p⁺ a alc4（xrs⁺或xrs1）×未照射的p⁻α ade2 ura（xrs⁺或xrs1）杂交后代中，分析了红色菌落的基因型：在大多数情况下（82.3% - 99.8%），它们是p⁺α ade2 ura，即真正的单倍体细胞融合体。得出的结论是，照射诱导酵母细胞核的特定损伤，导致正常核融合受阻，从而形成细胞融合体。在辐射敏感突变体的杂交中观察到了最高的细胞融合频率。
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