Brady Meghan J, Cheam Maya, Gent Jonathan I, Dawe R Kelly
Department of Genetics University of Georgia Athens Georgia USA.
Department of Plant Biology University of Georgia Athens Georgia USA.
Plant Direct. 2024 Feb 13;8(2):e567. doi: 10.1002/pld3.567. eCollection 2024 Feb.
Maize s () is a mutant that causes white stripes on leaves that has been used in mapping studies for decades though the underlying gene has not been identified. The locus has been previously mapped to small regions of normal chromosome 10 (N10) and a rearranged variant called abnormal chromosome 10 (Ab10). A comparison of assembled genomes carrying N10 and Ab10 revealed only five candidate genes. Analysis of a stock carrying the reference allele () showed that one of the five genes has a transposon insertion that disrupts its protein sequence and has a severe reduction in mRNA. An independent Mutator transposon insertion in the gene () failed to complement the mutation, and plants homozygous for showed white striped leaf margins. The gene encodes a DUF3732 protein with strong homology to a rice gene with a similar mutant phenotype called (). These and other published data suggest that may have a function in plastid gene expression.
玉米条纹叶(s)突变体可导致叶片出现白色条纹,尽管其潜在基因尚未确定,但已用于定位研究数十年。该位点先前已被定位到正常10号染色体(N10)的小区域以及一个名为异常10号染色体(Ab10)的重排变体上。对携带N10和Ab10的组装基因组进行比较,仅发现了五个候选s基因。对携带s参考等位基因(+)的品系进行分析表明,这五个基因中的一个存在转座子插入,破坏了其蛋白质序列,且mRNA严重减少。该基因(s)中的一个独立的Mutator转座子插入未能互补s突变,并且s纯合的植物表现出白色条纹叶缘。s基因编码一种DUF3732蛋白,与具有类似突变表型的水稻基因(vrs1)具有很强的同源性。这些以及其他已发表的数据表明,s可能在质体基因表达中具有功能。