微升液滴中的集成相分离用于超痕量生物标志物分析。
Integrated phase separation in microliter droplets for ultratrace-enriching biomarker analysis.
机构信息
College of Chemistry and Environmental Engineering, The Institute for Advanced Study (IAS), Shenzhen University, Shenzhen, Guangdong, 518055, P. R. China.
Beijing Key Laboratory for Bioengineering and Sensing Technology, School of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing, 100083, P. R. China.
出版信息
Lab Chip. 2024 Mar 12;24(6):1775-1781. doi: 10.1039/d3lc00953j.
Ultratrace-enriching biomarker analysis is an effective method for achieving highly accurate and enhanced sensitive detection. In this study, we have developed an enrichment detection platform by combining a minipillar array with an aqueous two-phase system (ATPS) for ultratrace enriching biomarker analysis. After optimizing the enrichment conditions of ATPS, target miRNAs at ultratrace levels specifically accumulate in the DEX-rich phase, which significantly increases the target miRNA concentration-related fluorescence intensity. Compared to non-enriched miRNA in the single-phase PEG solution, the detection limit of ATPS-enriched miRNA had improved more than 200-fold. The ATPS-based enrichment detection strategy offers a novel and convenient approach for the simultaneous detection of biomarkers with ultratrace.
超痕量富集生物标志物分析是实现高精准度和高灵敏度检测的有效方法。在本研究中，我们开发了一种基于 minipillar 阵列与双水相系统（ATPS）的富集检测平台，用于超痕量生物标志物的分析。在优化 ATPS 的富集条件后，特定的痕量目标 miRNA 会特异性地聚集在富含 DEX 的相中，从而显著提高了与目标 miRNA 浓度相关的荧光强度。与单相 PEG 溶液中非富集的 miRNA 相比，基于 ATPS 的 miRNA 富集检测的检测限提高了 200 多倍。基于 ATPS 的富集检测策略为超痕量生物标志物的同时检测提供了一种新颖、便捷的方法。
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