Department of Urology, The Third Affiliated Hospital of Jinzhou Medical University, Jinzhou, Liaoning, 121000, China.
Department of Urology, The Affiliated Hospital of Changchun University of Chinese Medicine, Changchun, China.
Curr Stem Cell Res Ther. 2024;19(10):1382-1392. doi: 10.2174/011574888X253599231126161254.
The aim of this study was to construct a multicompartment synchronous rotating bioreactor (MCSRB) for batch-production of homogenized adipose-derived stem cell (ADSC) microspheres and treat neurogenic erectile dysfunction (ED).
Firstly, an MCSRB was constructed using a centrifugal device and hinged trays. Secondly, influence factors (density, rotational speed) on the formation of ADSC-spheroids were explored. Finally, a neurogenic ED model was established to verify the effectiveness and safety of ADSC-spheroids for ED treatment.
An MCSRB promoted ADSCs to gather microspheres, most of which were 90-130 μm in diameter. Supernatant from three-dimensional culture led to a significant increase in cytokine expression in ADSCs and migration rate in human umbilical vein endothelial cells (HUVECs) compared to control groups. The erectile function and pathological changes of the penis were improved in the ADSC-spheroids treatment group compared to the traditional ADSCs treatment group (p < 0.01).
Efficient, batch, controlled and homogenized production of ADSC stem cell microspheres, and effective improvement of erectile dysfunction in neurogenic rats can be achieved using the MCSRB device.
本研究旨在构建一种多隔室同步旋转生物反应器（MCSRB），用于批量生产均质脂肪来源干细胞（ADSC）微球，并治疗神经性勃起功能障碍（ED）。
首先，使用离心装置和铰链托盘构建 MCSRB。其次，探索影响 ADSC 球形成的因素（密度、转速）。最后，建立神经性 ED 模型，验证 ADSC 微球治疗 ED 的有效性和安全性。
MCSRB 促进 ADSCs 聚集形成微球，其中大多数直径为 90-130μm。与对照组相比，三维培养的上清液导致 ADSCs 中细胞因子表达和人脐静脉内皮细胞（HUVEC）迁移率显著增加。与传统 ADSCs 治疗组相比，ADSC 微球治疗组的勃起功能和阴茎病理变化得到改善（p<0.01）。
使用 MCSRB 装置可高效、批量、可控、均质地生产 ADSC 干细胞微球，并有效改善神经性大鼠的勃起功能障碍。
