Department of Pathology, Kalinga Institute of Medical Sciences, Bhubaneswar, Odisha, India.
Department of Surgical Oncology, Kalinga Institute of Medical Sciences, Bhubaneswar, Odisha, India.
Indian J Pathol Microbiol. 2024 Jan-Mar;67(1):15-20. doi: 10.4103/ijpm.ijpm_535_22.
With no unified system for tumor associated macrophages (TAMs) density assessment, limited information is available on their relationship with β-catenin expression.
To evaluate the density of CD68 TAMs in gastric adenocarcinoma samples by immunohistochemistry and correlate it with grade, stage, invasion, and beta-catenin.
Formalin fixed paraffin embedded (FFPE) blocks from gastrectomy specimens of proven gastric adenocarcinoma were prospectively and retrospectively were studied over a period of two years.
Immunohistochemistry with CD68 and β-catenin was performed. TAM density was qualitatively compared in "tumor" versus "stroma" and "tumor" versus "non-tumor" regions. Quantitative CD68 TAM density was assessed using different methods and compared. Cases were classified as high and low TAM based on the median value and correlated with histologic type, location, grade, stage and β-catenin expression pattern.
Spearman's rank correlation test was used to compare the different methods of TAM density evaluation. The categorical variables were studied using Pearson's Chi-square or Fisher's exact test. CD68 TAM density and β-catenin expression were correlated by analysis of variance. A P value ≤ 0.05 was taken as statistically significant.
The CD68 TAMs in the "tumor" versus "non-tumor" area (p = 0.34) and "tumor" versus "stroma distribution" (p = 0.81) did not show any statistical significance. All methods of TAM density were found to be comparable. High TAM group is significantly associated with lymphovascular invasion, tumor depth, lymph node metastasis, and abnormal β-catenin expression.
TAMs density plays an important role in the tumor stage. Macrophages may possibly induce gastric cancer invasiveness by activating β-catenin pathway.
由于缺乏肿瘤相关巨噬细胞（TAMs）密度评估的统一系统，关于它们与β-连环蛋白表达的关系的信息有限。
通过免疫组织化学评估胃腺癌样本中 CD68 TAMs 的密度，并将其与分级、分期、浸润和β-连环蛋白相关联。
对两年内前瞻性和回顾性研究的胃腺癌切除术标本的福尔马林固定石蜡包埋（FFPE）块进行了研究。
进行了 CD68 和 β-连环蛋白的免疫组织化学染色。在“肿瘤”与“基质”和“肿瘤”与“非肿瘤”区域之间定性比较 TAM 密度。使用不同的方法评估定量 CD68 TAM 密度并进行比较。根据中位数将病例分为高 TAM 和低 TAM，并与组织学类型、位置、分级、分期和β-连环蛋白表达模式相关联。
使用 Spearman 等级相关检验比较 TAM 密度评估的不同方法。使用 Pearson 卡方或 Fisher 确切检验研究分类变量。通过方差分析对 CD68 TAM 密度和β-连环蛋白表达进行相关性分析。P 值≤0.05 被认为具有统计学意义。
“肿瘤”与“非肿瘤”区域（p=0.34）和“肿瘤”与“基质分布”（p=0.81）的 CD68 TAMs 之间没有统计学意义。所有 TAM 密度方法均具有可比性。高 TAM 组与血管淋巴管浸润、肿瘤深度、淋巴结转移和异常β-连环蛋白表达显著相关。
TAMs 密度在肿瘤分期中起重要作用。巨噬细胞可能通过激活β-连环蛋白通路诱导胃癌侵袭。
