Department of Analytical and Bioanalytical Chemistry, School of Pharmaceutical Sciences, University of Shizuoka, 52-1, Yada, Suruga-ku, Shizuoka, 422-8526, Japan.
Laboratory of Analytical Chemistry, Faculty of Pharmacy, Meijo University, 150 Yagotoyama, Tempaku, Nagoya, 468-8503, Japan.
Anal Sci. 2024 May;40(5):863-869. doi: 10.1007/s44211-024-00513-x. Epub 2024 Feb 15.
We report on the development of an on-site therapeutic drug monitoring (TDM) method for vancomycin (VCM) utilizing a portable spectrometer and commercially available immunoturbidimetric assay reagents designed for automated clinical chemistry analyzers. The method enables the quantification of VCM in plasma samples within 10 min, with a good correlation between the measured values and the theoretical values (r = 0.995). The intra and inter-day precisions were found to be below 12.5% and 17.7%, respectively. Moreover, we established a correlation between the quantitative values using this method and those measured through HPLC-UV and automated clinical chemistry analyzers, showing good reliability (R = 0.970 and 0.951, respectively). This method allows anyone to rapidly perform TDM at the bedside and is expected to be used to evaluate appropriate drug therapy.
我们报告了一种利用便携式分光光度计和商业上可用于自动化临床化学分析仪的免疫比浊测定试剂开发的用于万古霉素（VCM）的现场治疗药物监测（TDM）方法。该方法能够在 10 分钟内定量测定血浆样品中的 VCM，测量值与理论值之间具有良好的相关性（r=0.995）。日内和日间精密度分别低于 12.5%和 17.7%。此外，我们还建立了使用该方法的定量值与通过 HPLC-UV 和自动化临床化学分析仪测量的值之间的相关性，显示出良好的可靠性（分别为 R=0.970 和 0.951）。该方法允许任何人在床边快速进行 TDM，并有望用于评估适当的药物治疗。
