Instituto de Investigaciones en Ciencias de la Salud (INICSA), CONICET and Cátedra de Bioquímica y Biología Molecular, Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, Pabellón Argentina 2do Piso, Córdoba 5000, Argentina.
Instituto de Investigaciones en Ciencias de la Salud (INICSA), CONICET and Cátedra de Bioquímica y Biología Molecular, Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, Pabellón Argentina 2do Piso, Córdoba 5000, Argentina.
Acta Trop. 2024 Apr;252:107149. doi: 10.1016/j.actatropica.2024.107149. Epub 2024 Feb 13.
The enzyme NADPH-cytochrome P450 reductase (CPR) plays a central role in cytochromes P450 activity. Gene expression analysis of cytochromes P450 and CPR in deltamethrin-resistant and susceptible populations revealed that P450s genes are involved in the development of insecticide resistance in Triatoma infestans. To clarify the role of cytochromes P450 in insecticide resistance, it was proposed to investigate the effect of CPR gene silencing by RNA interference (RNAi) in a pyrethroid resistant population of T. infestans. Silencing of the CPR gene showed a significant increase in susceptibility to deltamethrin in the population analysed. This result support the hypothesis that the metabolic process of detoxification mediated by cytochromes P450 contributes to the decreased deltamethrin susceptibility observed in the resistant strain of T. infestans.
NADPH-细胞色素 P450 还原酶（CPR）是细胞色素 P450 活性的核心酶。对溴氰菊酯抗性和敏感种群细胞色素 P450 和 CPR 的基因表达分析表明，P450 基因参与了三带喙库蚊对杀虫剂的抗性发展。为了阐明细胞色素 P450 在杀虫剂抗性中的作用，拟通过 RNA 干扰（RNAi）研究 CPR 基因沉默对三带喙库蚊拟除虫菊酯抗性种群的影响。在分析的种群中，CPR 基因沉默显著增加了对溴氰菊酯的敏感性。这一结果支持了这样一种假设，即细胞色素 P450 介导的解毒代谢过程导致了三带喙库蚊抗性品系对溴氰菊酯敏感性降低。
