Anderson R L, Lunec J, Cresswell S R
Int J Hyperthermia. 1985 Oct-Dec;1(4):337-47. doi: 10.3109/02656738509029297.
The cholesterol, phospholipid and protein contents of nine mammalian cell lines, three lymphoid and six attached cell lines, were measured, along with the sensitivity of the cells to hyperthermia at 42 degrees and 44 degrees C. The free cholesterol content and the protein content per cell correlated positively with the time required to kill 99 per cent of the cells at 44 degrees C. The phospholipid content showed a less significant positive correlation whilst the cholesterol ester content and the cholesterol:phospholipid molar ratio did not correlate with heat sensitivity. There were no correlations observed when the levels of these cell components were compared to heat survival at 42 degrees C. As the three lymphoid lines are small, very heat sensitive cells, the six monolayer lines were analysed separately. In this case, only the protein and the free cholesterol content maintained a significant correlation (at the 5 per cent level). It is concluded that the levels of cholesterol or phospholipid cannot be used as reliable indicators of the heat sensitivity of a cell.
对9种哺乳动物细胞系（3种淋巴样细胞系和6种贴壁细胞系）的胆固醇、磷脂和蛋白质含量进行了测定，并检测了这些细胞在42℃和44℃时对热疗的敏感性。每个细胞的游离胆固醇含量和蛋白质含量与在44℃下杀死99%细胞所需的时间呈正相关。磷脂含量呈现出不太显著的正相关，而胆固醇酯含量以及胆固醇与磷脂的摩尔比与热敏感性无关。当将这些细胞成分的水平与42℃时的热存活率进行比较时，未观察到相关性。由于这3种淋巴样细胞系是体积小、对热非常敏感的细胞，因此对6种单层细胞系进行了单独分析。在这种情况下，只有蛋白质和游离胆固醇含量保持显著相关性（在5%水平）。得出的结论是，胆固醇或磷脂的水平不能用作细胞热敏感性的可靠指标。
