Mycotic Diseases Branch, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.
Washington State Department of Health, State Public Health Agency, Shoreline, Washington, USA.
Microbiol Spectr. 2024 Apr 2;12(4):e0356423. doi: 10.1128/spectrum.03564-23. Epub 2024 Feb 16.
CHROMagar Plus is a new formulation of chromogenic media designed for the detection and differentiation of major clinical species, including . The objective of this study is to evaluate CHROMagar Plus when used according to manufacturer's instructions with a panel of 206 fungal isolates and 83 skin-swab specimens originally collected for colonization screening. Of the 68 . isolates tested, 66/68 displayed the expected light-blue colony morphology and blue halo within 48 h. None of the remaining 138 non- isolates appeared similar to . CHROMagar Plus was, therefore, inclusive to 97% of 68 . isolates tested and supported visual exclusion of 100% of the 138 non-. isolates tested. For the 83 colonization screening specimens, direct plating onto CHROMagar Plus was 60% sensitive and 100% specific when compared to the enrichment broth gold-standard reference method. In sum, these findings demonstrate the utility of this media when working with isolates but also notable limitations when working with primary skin-swabs specimens when competing yeast species are present.IMPORTANCE is an emerging fungal pathogen of public health concern. As it continues to spread, it is important to publish evaluations of new diagnostic tools. In this study, we share our experience with a new chromogenic media which can help distinguish from related species.
CHROMagar Plus 是一种新型显色培养基，专为检测和区分主要临床物种而设计，包括 。本研究的目的是评估 CHROMagar Plus 在按照制造商的说明使用时，对 206 株真菌分离株和 83 份皮肤拭子标本的检测效果，这些分离株和标本最初是为了定植筛查而收集的。在 68 株 中，66/68 在 48 小时内显示出预期的浅蓝色菌落形态和蓝色晕圈。其余 138 株非 分离株中没有一株表现出类似的形态。因此，CHROMagar Plus 对 68 株 测试分离株的包容性达到 97%，并支持对 138 株非 测试分离株的 100%视觉排除。对于 83 份定植筛查标本，与富集肉汤金标准参考方法相比，直接接种到 CHROMagar Plus 上的敏感性为 60%，特异性为 100%。总之，这些发现表明，该培养基在处理分离株时具有实用性，但在处理存在竞争酵母物种的原发性皮肤拭子标本时，也存在显著的局限性。 是一种具有公共卫生意义的新兴真菌病原体。随着它的传播，发布新的诊断工具的评估结果非常重要。在这项研究中，我们分享了我们使用一种新的显色培养基的经验，这种培养基可以帮助区分 与相关物种。
