Institute of Medical Mycology, Teikyo University, Hachioji, Tokyo, Japan.
Microbiol Immunol. 2022 Jun;66(6):292-298. doi: 10.1111/1348-0421.12973. Epub 2022 Apr 7.
Skin colonization by the emerging pathogen Candida auris is common in outbreaks within medical settings. Culture-based screening of patients is an effective management strategy to control the pathogen, and the newly developed CHROMagar™ Candida Plus medium is claimed to enable the presumptive identification of C. auris. Here, we evaluated the use of this medium with 63 C. auris strains comprising its four well-established clades, as well as genetically related comparators, including species from the Metschnikowia clade. The colors and halos of both confluent growth and discrete colonies of all the tested strains were compared. It was found that on CHROMagar™ Candida Plus, C. auris formed characteristic white colonies with blue-green halos that were more evident after 72 hr of incubation at 35°C than after 48 hr. However, distinguishing between closely related species such as Candida haemulonii, Candida pseudohaemulonii, and Candida duobushaemulonii required the consideration of parameters other than color, including colony size and growth ability at 35°C. In conclusion, the novel chromogenic medium CHROMagar™ Candida Plus constitutes an easy screening tool for C. auris.
皮肤定植是新兴病原体耳念珠菌在医疗环境中暴发的常见现象。对患者进行基于培养的筛查是控制病原体的有效管理策略，新开发的 CHROMagar™ Candida Plus 培养基据称能够对耳念珠菌进行初步鉴定。在这里，我们评估了该培养基对包括其四个已确立分支在内的 63 株耳念珠菌菌株的使用情况，以及遗传上相关的对照物，包括来自 Metschnikowia 分支的物种。比较了所有测试菌株的融合生长和离散菌落的颜色和晕圈。结果发现，在 CHROMagar™ Candida Plus 上，耳念珠菌形成具有蓝绿色晕圈的特征白色菌落，在 35°C 孵育 72 小时后比 48 小时后更明显。然而，要区分密切相关的物种，如溶血假丝酵母、拟溶血假丝酵母和双头假丝酵母，除了颜色之外，还需要考虑其他参数，包括菌落大小和在 35°C 下的生长能力。总之，新型显色培养基 CHROMagar™ Candida Plus 是一种用于耳念珠菌的简单筛选工具。
