Mulet Bayona Juan Vicente, Salvador García Carme, Tormo Palop Nuria, Valentín Martín Amparo, González Padrón Carmelo, Colomina Rodríguez Javier, Pemán Javier, Gimeno Cardona Concepción
Department of Microbiology and Parasitology, Consorcio Hospital General Universitario de Valencia, 46014 Valencia, Spain.
Department of Microbiology and Parasitology, Hospital Universitari I Politècnic La Fe de Valencia, 46026 Valencia, Spain.
J Fungi (Basel). 2022 Mar 9;8(3):281. doi: 10.3390/jof8030281.
Epidemiological trends show a dramatic increase in the prevalence of fungal infections, and in the isolation of multidrug-resistant species, such as CHROMagar Candida (CC; CHROMagar, Paris, France) and other chromogenic media, which are widely used in the clinical laboratory because they allow a rapid identification of most species. Recently, CHROMagar Candida Plus (CC-Plus; CHROMagar, Paris, France) was developed to detect and differentiate in addition to other major clinical species, such as , . , , or display a differential light blue color with a blue halo. A multicentric study was designed to evaluate the performance of the CC-Plus medium in the detection of species in patients' surveillance and environmental samples from three Spanish hospitals with active outbreaks. A total of 364 patients' surveillance samples and 212 environmental samples were tested. Samples were inoculated in CC and CC-Plus in parallel, and the plates were read at 24 and 48 h. All recovered colonies were presumptively identified according to colony color described by manufacturer, and the definitive identification was performed by mass spectrometry at 48 h. A total of 134 isolates were obtained (101 from patients' surveillance samples, and 33 from environmental samples). Sensitivity, specificity, and predictive positive and negative values were 99.5%, 100%, 100%, and 99.1%, respectively, for the main clinical species, showing that CC-Plus is comparable to CC, with the advantage of being able to differentiate from . Furthermore, CC-Plus was able to detect one , one , and eight that did not grow in CC. Additionally, the yeast colonies were generally larger, suggesting that this novel medium could be a richer medium, and suitable for surveillance and environmental cultures of and other clinically relevant species.
流行病学趋势显示真菌感染的患病率急剧上升，耐多药菌种的分离情况亦是如此，比如显色培养基念珠菌（CC；法国巴黎科玛嘉公司）和其他显色培养基，它们在临床实验室中被广泛使用，因为能够快速鉴定出大多数菌种。最近，科玛嘉念珠菌增强型培养基（CC-Plus；法国巴黎科玛嘉公司）被研发出来，除了能检测和区分其他主要临床菌种外，比如…… ，…… ，…… ，…… 或…… 呈现出带蓝色晕圈的浅蓝色。一项多中心研究旨在评估CC-Plus培养基在检测来自西班牙三家有活跃…… 疫情医院的患者监测样本和环境样本中的…… 菌种时的性能。总共检测了364份患者监测样本和212份环境样本。样本同时接种于CC和CC-Plus培养基中，平板在24小时和48小时时进行读数。所有回收的菌落根据制造商描述的菌落颜色进行初步鉴定，最终鉴定在48小时时通过质谱法进行。总共获得了134株…… 分离株（101株来自患者监测样本，33株来自环境样本）。对于主要临床…… 菌种，敏感性、特异性以及预测阳性和阴性值分别为99.5%、100%、100%和99.1%，表明CC-Plus与CC相当，其优势在于能够区分…… 和…… 。此外，CC-Plus能够检测出在CC中未生长的1株…… 、1株…… 和8株…… 。另外，酵母菌落通常更大，这表明这种新型培养基可能是一种营养更丰富的培养基，适用于…… 和其他临床相关…… 菌种的监测和环境培养。
