Department of Chemistry, College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, PR China; Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, State Key Laboratory of Applied Microbiology Southern China, Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, PR China.
Department of Chemistry, College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, PR China.
Colloids Surf B Biointerfaces. 2024 Mar;235:113784. doi: 10.1016/j.colsurfb.2024.113784. Epub 2024 Feb 5.
Recently, researches focused towards the chiral nanostructures have attracted vast attention. However, the synthesis of chiral carbon dots (CDs) through one-step method is still rather scarce. Herein, a universal approach to green synthesis of chiral CDs at low temperature was proposed. In brief, L-FruCDs and D-FruCDs were obtained by only heating the fructose and chiral cysteine molecules in the sodium hydroxide aqueous solution under atmospheric pressure. Circular dichroism spectra show that these prepared CDs exhibit opposite chirality ranging from 210 to 260 nm. Specially, the prepared L-FruCDs could reduce the intracellular oxidative damage induced by hydrogen peroxide and display a superior performance than that of D-FruCDs. Mechanism studies indicate that the probably protect mechanism is ascribed to the directly consumption the intracellular ROS. And the clearance efficiency of intracellular reactive oxygen species of L-FruCDs is 3-times than that of D-FruCDs. Furthermore, this newly synthesized method is scalable by replacing fructose precursor with ascorbic acid, sucrose or lactose. In sum, our work provides a new method for the preparation of chiral CDs and achieve a great success in exploring the chiral biological effects at nanoscale.
近年来，对手性纳米结构的研究引起了广泛关注。然而，通过一步法合成手性碳点（CDs）仍然相当稀缺。在此，提出了一种在低温下通过一步法绿色合成手性 CDs 的通用方法。简而言之，通过在常压下将果糖和手性半胱氨酸分子在氢氧化钠水溶液中加热，得到 L-FruCDs 和 D-FruCDs。圆二色光谱表明，这些制备的 CDs 在 210 到 260nm 范围内表现出相反的手性。特别是，所制备的 L-FruCDs 可以减轻由过氧化氢诱导的细胞内氧化损伤，并且性能优于 D-FruCDs。机制研究表明，可能的保护机制归因于直接消耗细胞内的 ROS。并且 L-FruCDs 对细胞内活性氧的清除效率是 D-FruCDs 的 3 倍。此外，通过用抗坏血酸、蔗糖或乳糖代替果糖前体，这种新合成的方法可以扩展规模。总之，我们的工作为手性 CDs 的制备提供了一种新方法，并在手性生物效应的纳米尺度探索方面取得了巨大成功。
