从半胱氨酸中提取的高荧光手性 N-S 掺杂碳点：影响细胞能量代谢。
Highly Fluorescent Chiral N-S-Doped Carbon Dots from Cysteine: Affecting Cellular Energy Metabolism.
机构信息
Key Lab of Organic Optoelectronics & Molecular Engineering, Department of Chemistry, Tsinghua University, Beijing, 100084, P. R. China.
School of Chemical Engineering and Technology, Key Laboratory of Systems Bioengineering (Ministry of Education), Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin University, Tianjin, 300072, P. R. China.
出版信息
Angew Chem Int Ed Engl. 2018 Feb 23;57(9):2377-2382. doi: 10.1002/anie.201712453. Epub 2018 Feb 8.
Cysteine-based chiral optically active carbon dots (CDs) and their effects on cellular energy metabolism, which is vital for essential cellular functions, have been barely reported. A green and effective synthesis strategy for chiral N-S-doped CDs (fluorescence quantum yield ca. 41.26 %) based on hydrothermal treatment of l- or d-cysteine at as low as 60 °C has been developed. This suggested that cysteine was instable in aqueous solutions and acts as a warning for high-temperature synthesis of nanomaterials using cysteine as stabilizer. Human bladder cancer T24 cells treated with l-CDs showed up-regulated glycolysis, while d-CDs had no similar effects. In contrast, no disturbance to the basal mitochondrial aerobic respiration of T24 cells was caused by either chiral CD.
基于半胱氨酸的手性光学活性碳点（CDs）及其对细胞能量代谢的影响，这对基本的细胞功能至关重要，但几乎没有报道。本文开发了一种绿色有效的手性 N-S 掺杂 CDs（荧光量子产率约为 41.26%）的合成策略，该策略是通过在低至 60°C 的条件下水热处理 l-或 d-半胱氨酸得到的。这表明半胱氨酸在水溶液中不稳定，并对使用半胱氨酸作为稳定剂高温合成纳米材料发出警告。用 l-CDs 处理的人膀胱癌 T24 细胞表现出糖酵解增强，而 d-CDs 则没有类似的作用。相比之下，手性 CD 对 T24 细胞的基础线粒体需氧呼吸没有干扰。
Zotero 插件