谷胱甘肽 S-转移酶活性促进水稻对稗草根分泌物 DIMBOA 的耐受性。

Glutathione S-transferase activity facilitates rice tolerance to the barnyard grass root exudate DIMBOA.

机构信息

Fujian Provincial Key Laboratory of Agroecological Processing and Safety Monitoring, College of JunCao Science and Ecology, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.

Institute of Oceanography, College of Geography and Oceanography, Minjiang University, Fuzhou, 350108, China.

出版信息

BMC Plant Biol. 2024 Feb 17;24(1):117. doi: 10.1186/s12870-024-04802-5.

DOI:10.1186/s12870-024-04802-5

PMID:38365588

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10874003/

Abstract

BACKGROUND

In paddy fields, the noxious weed barnyard grass secretes 2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one (DIMBOA) to interfere with rice growth. Rice is unable to synthesize DIMBOA. Rice cultivars with high or low levels of allelopathy may respond differently to DIMBOA.

RESULTS

In this study, we found that low concentrations of DIMBOA (≤ 0.06 mM) promoted seedling growth in allelopathic rice PI312777, while DIMBOA (≤ 0.08 mM) had no significant influence on the nonallelopathic rice Lemont. DIMBOA treatment caused changes in the expression of a large number of glutathione S-transferase (GST) proteins, which resulting in enrichment of the glutathione metabolic pathway. This pathway facilitates plant detoxification of heterologous substances. The basal levels of GST activity in Lemont were significantly higher than those in PI312777, while GST activity in PI312777 was slightly induced by increasing DIMBOA concentrations. Overexpression of GST genes (Os09g0367700 and Os01g0949800) in these two cultivars enhanced rice resistance to DIMBOA.

CONCLUSIONS

Taken together, our results indicated that different rice accessions with different levels of allelopathy have variable tolerance to DIMBOA. Lemont had higher GST activity, which helped it tolerate DIMBOA, while PI312777 had lower GST activity that was more inducible. The enhancement of GST expression facilitates rice tolerance to DIMBOA toxins from barnyard grass root exudates.

摘要

背景

在稻田中，恶性杂草稗草会分泌 2,4-二羟基-7-甲氧基-2H-1,4-苯并恶嗪-3(4H)-酮（DIMBOA）来干扰水稻的生长。水稻无法合成 DIMBOA。具有高或低化感作用的水稻品种可能对 DIMBOA 有不同的反应。

结果

在这项研究中，我们发现低浓度的 DIMBOA（≤0.06 mM）促进了化感水稻 PI312777 的幼苗生长，而 DIMBOA（≤0.08 mM）对非化感水稻 Lemont 没有显著影响。DIMBOA 处理导致大量谷胱甘肽 S-转移酶（GST）蛋白的表达发生变化，从而丰富了谷胱甘肽代谢途径。该途径有助于植物对外源物质的解毒。Lemont 的 GST 活性基础水平明显高于 PI312777，而 PI312777 中的 GST 活性则随着 DIMBOA 浓度的增加而略有诱导。这两个品种中 GST 基因（Os09g0367700 和 Os01g0949800）的过表达增强了水稻对 DIMBOA 的抗性。