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暴露于60赫兹电场中的蚕豆根的生长速率和有丝分裂指数分析。

Growth rate and mitotic index analysis of Vicia faba L. roots exposed to 60-Hz electric fields.

作者信息

Inoue M, Miller M W, Cox C, Carstesen E L

出版信息

Bioelectromagnetics. 1985;6(3):293-303. doi: 10.1002/bem.2250060309.

DOI:10.1002/bem.2250060309
PMID:3836672
Abstract

Growth, mitotic index, and growth rate recovery were determined for Vicia faba L. roots exposed to 60-Hz electric fields of 200, 290, and 360 V/m in an aqueous inorganic nutrient medium (conductivity 0.07-0.09 S/m). Root growth rate decreased in proportion to the increasing strength; the electric field threshold for a growth rate effect was about 230 V/m. The induced transmembrane potential at the threshold exposure was about 4-7 mV. The mitotic index was not affected by an electric field exposure sufficient to reduce root growth rate to about 35% of control. Root growth rate recovery from 31-96% of control occurred in 4 days after cessation of the 360 V/m exposure. The results support the postulate that the site of action of the applied electric fields is the cell membrane.

摘要

在无机营养水溶液培养基(电导率0.07 - 0.09 S/m)中,测定了暴露于200、290和360 V/m的60 Hz电场下的蚕豆根的生长、有丝分裂指数和生长速率恢复情况。根生长速率随电场强度增加而呈比例下降;生长速率效应的电场阈值约为230 V/m。阈值暴露时诱导的跨膜电位约为4 - 7 mV。有丝分裂指数不受足以将根生长速率降低至对照约35%的电场暴露影响。在360 V/m暴露停止后的4天内,根生长速率从对照的31% - 96%恢复。结果支持所施加电场的作用位点是细胞膜这一假设。

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