Bacterial Diseases Branch, Division of Vector-Borne Diseases, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, Fort Collins, CO, USA.
Bacterial Diseases Branch, Division of Vector-Borne Diseases, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, Fort Collins, CO, USA.
Ticks Tick Borne Dis. 2024 May;15(3):102324. doi: 10.1016/j.ttbdis.2024.102324. Epub 2024 Feb 16.
A Borrelia miyamotoi gene with partial homology to bipA of relapsing fever spirochetes Borrelia hermsii and Borrelia turicatae was identified by a GenBank basic alignment search analysis. We hypothesized that this gene product may be an immunogenic antigen as described for other relapsing fever Borrelia (RFB) and could serve as a serological marker for B. miyamotoi infections. The B. miyamotoi gene was a truncated version about half the size of the B. hermsii and B. turicatae bipA with a coding sequence of 894 base pairs. The gene product had a calculated molecular size of 32.7 kDa (including the signal peptide). Amino acid alignments with B. hermsii and B. turicatae BipA proteins and with other B. miyamotoi isolates showed conservation at the carboxyl end. We cloned the B. miyamotoi bipA-like gene (herein named bipM) and generated recombinant protein for serological characterization and for antiserum production. Protease protection analysis demonstrated that BipM was surface exposed. Serologic analyses using anti-B. miyamotoi serum samples from tick bite-infected and needle inoculated mice showed 94 % positivity against BipM. The 4 BipM negative serum samples were blotted against another B. miyamotoi antigen, BmaA, and two of them were seropositive resulting in 97 % positivity with both antigens. Serum samples from B. burgdorferi sensu stricto (s.s.)-infected mice were non-reactive against rBipM by immunoblot. Serum samples from Lyme disease patients were also serologically negative against BipM except for 1 sample which may have indicated a possible co-infection. A recently published study demonstrated that B. miyamotoi BipM was non-reactive against serum samples from B. hermsii, Borrelia parkeri, and B. turicatae infected animals. These results show that BipM has potential for a B. miyamotoi-infection specific and sensitive serodiagnostic to differentiate between Lyme disease and various RFB infections.
通过 GenBank 基本比对搜索分析，发现一种与回归热螺旋体博氏疏螺旋体和特氏疏螺旋体 bipA 部分同源的米氏包柔螺旋体基因。我们假设该基因产物可能像其他回归热包柔螺旋体（RFB）一样是一种免疫原性抗原，并可作为米氏包柔螺旋体感染的血清学标志物。该米氏包柔螺旋体基因是一个截断版本，大小约为博氏疏螺旋体和特氏疏螺旋体 bipA 的一半，编码序列为 894 个碱基对。该基因产物的计算分子大小为 32.7 kDa（包括信号肽）。与博氏疏螺旋体和特氏疏螺旋体 BipA 蛋白以及其他米氏包柔螺旋体分离株的氨基酸比对显示羧基末端保守。我们克隆了米氏包柔螺旋体 bipA 样基因（在此命名为 bipM），并生成了重组蛋白用于血清学特征分析和抗血清生产。蛋白酶保护分析表明 BipM 是表面暴露的。使用来自蜱叮咬感染和针接种感染的小鼠的抗米氏包柔螺旋体血清样本进行的血清学分析显示，94%的样本对 BipM 呈阳性。4 份 BipM 阴性血清样本与另一种米氏包柔螺旋体抗原 BmaA 杂交，其中 2 份血清样本呈阳性，这两种抗原的阳性率均为 97%。来自伯氏疏螺旋体严格意义上（s.s.）感染的小鼠的血清样本通过免疫印迹对 rBipM 无反应。莱姆病患者的血清样本也对 BipM 呈血清学阴性，除了 1 份样本可能表明可能存在合并感染。最近的一项研究表明，米氏包柔螺旋体 BipM 对感染博氏疏螺旋体、帕克氏疏螺旋体和特氏疏螺旋体的动物的血清样本无反应。这些结果表明，BipM 具有针对米氏包柔螺旋体感染的特异性和敏感性血清学诊断潜力，可用于区分莱姆病和各种回归热包柔螺旋体感染。
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