研究核糖核酸聚合酶组分相互作用的新工具：利福平依赖性突变体。
New tool for studying interactions of components of ribonucleic acid polymerase: rifampin-dependent mutants.
作者信息
Dabbs E R
出版信息
J Bacteriol. 1979 Sep;139(3):1072-4. doi: 10.1128/jb.139.3.1072-1074.1979.
Abstract
Mutants of Escherichia coli showing a rifampin-dependent phenotype were isolated from cells of strain CP78 mutagenized with ethyl methane sulfonate or nitrosoguanidine when an antibiotic underlay technique was used. The mutants varied greatly in their rifampin requirement. The minimum necessary concentration ranged from 1 to 50 micrograms/ml. The mutants could be divided into four phenotypic classes. These dependent mutants and their revertants should be a useful tool for probing interactions between the component polypeptides of ribonucleic acid polymerase and for studying the linkage of transcription with other cellular processes.
摘要
当采用抗生素底层技术时，从用甲磺酸乙酯或亚硝基胍诱变的CP78菌株细胞中分离出了表现出利福平依赖性表型的大肠杆菌突变体。这些突变体对利福平的需求差异很大。所需的最低浓度范围为1至50微克/毫升。这些突变体可分为四个表型类别。这些依赖性突变体及其回复体应该是探究核糖核酸聚合酶组成多肽之间相互作用以及研究转录与其他细胞过程联系的有用工具。
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