State Key Laboratory of Vegetable Biobreeding, Sino-Dutch Joint Laboratory of Horticultural Genomics, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
Department of Plant Science and Landscape Architecture, University of Connecticut, Storrs, CT, 06269, USA.
Nat Commun. 2024 Feb 17;15(1):1472. doi: 10.1038/s41467-024-45722-0.
Understanding how plants alter their development and architecture in response to ambient temperature is crucial for breeding resilient crops. Here, we identify the quantitative trait locus qMULTIPLE INFLORESCENCE BRANCH 2 (qMIB2), which modulates inflorescence branching in response to high ambient temperature in tomato (Solanum lycopersicum). The non-functional mib2 allele may have been selected in large-fruited varieties to ensure larger and more uniform fruits under varying temperatures. MIB2 gene encodes a homolog of the Arabidopsis thaliana transcription factor SPATULA; its expression is induced in meristems at high temperature. MIB2 directly binds to the promoter of its downstream gene CONSTANS-Like1 (SlCOL1) by recognizing the conserved G-box motif to activate SlCOL1 expression in reproductive meristems. Overexpressing SlCOL1 rescue the reduced inflorescence branching of mib2, suggesting how the MIB2-SlCOL1 module helps tomato inflorescences adapt to high temperature. Our findings reveal the molecular mechanism underlying inflorescence thermomorphogenesis and provide a target for breeding climate-resilient crops.
了解植物如何响应环境温度改变其发育和结构对于培育具有弹性的作物至关重要。在这里，我们鉴定了数量性状位点 qMULTIPLE INFLORESCENCE BRANCH 2（qMIB2），它可以调节番茄（Solanum lycopersicum）花序分枝对环境温度的响应。非功能的 mib2 等位基因可能在大果品种中被选择，以确保在不同温度下果实更大且更均匀。MIB2 基因编码拟南芥转录因子 SPATULA 的同源物；其表达在高温下的分生组织中被诱导。MIB2 通过识别保守的 G-Box 基序直接与下游基因 CONSTANS-Like1（SlCOL1）的启动子结合，激活生殖分生组织中 SlCOL1 的表达。过表达 SlCOL1 挽救了 mib2 减少的花序分枝，表明 MIB2-SlCOL1 模块如何帮助番茄花序适应高温。我们的发现揭示了花序热形态发生的分子机制，并为培育具有气候弹性的作物提供了一个目标。
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