Key Laboratory of Silkworm and Bee Resource Utilization and Innovation of Zhejiang Province, College of Animal Sciences, Zhejiang University, Hangzhou 310058, China.
Key Laboratory of Silkworm and Bee Resource Utilization and Innovation of Zhejiang Province, College of Animal Sciences, Zhejiang University, Hangzhou 310058, China.
Ultrason Sonochem. 2024 Mar;104:106802. doi: 10.1016/j.ultsonch.2024.106802. Epub 2024 Feb 13.
Fatty acids are the key active components in royal jelly (RJ) with various biological activities. In this study, a novel ultrasound-assisted extraction (UAE) method was established to extract fatty acids from RJ and their structural and antioxidant property were further evaluated. The optimum extraction conditions were as follows: liquid-to-solid ratio of 10:1, ultrasonic power of 450 W and ultrasonic duration of 20 min, resulting in a better extraction yield of 16.48 % and 10-hydroxy-2(E)-decenoic acid (10-HDA) content of 4.12 %. Furthermore, compared with the solvent extraction method, the antioxidant activity of extract by ultrasound was enhanced significantly by at least 448 %. GC-MS showed that ultrasound didn't change the chemical composition of fatty acids, while it significantly increased the content of fatty acids. SEM image illustrated that extracts by UAE showed a rougher, looser microstructure compared to the solvent method. Overall, UAE is a promising method to obtain fatty acids in RJ with high efficiency.
脂肪酸是蜂王浆（RJ）中的主要活性成分，具有多种生物活性。本研究建立了一种新型超声辅助提取（UAE）方法，从 RJ 中提取脂肪酸，并进一步评估其结构和抗氧化性能。最佳提取条件为：液固比 10:1、超声功率 450 W 和超声时间 20 min，提取率为 16.48%，10-羟基-2(E)-癸烯酸（10-HDA）含量为 4.12%。此外，与溶剂提取法相比，超声提取的抗氧化活性至少提高了 448%。GC-MS 表明，超声并没有改变脂肪酸的化学成分，只是显著增加了脂肪酸的含量。SEM 图像表明，与溶剂法相比，UAE 提取的脂肪酸结构更粗糙、更疏松。总的来说，UAE 是一种从 RJ 中高效获得脂肪酸的有前途的方法。
