Ma Lili, Zheng Yanyan, Zhou Zhongjing, Deng Zhiping, Tan Jinjuan, Bai Chunmei, Fu Anzhen, Wang Qing, Zuo Jinhua
Institute of Agri-food Processing and Nutrition, Beijing Academy of Agriculture and Forestry Sciences, Beijing Key Laboratory of Fruits and Vegetable Storage and Processing, Key Laboratory of Vegetable Postharvest Processing of Ministry of Agriculture and Rural Areas, State Key Laboratory of Vegetable Biobreeding, Beijing Vegetable Research Center, Beijing Academy of Agriculture and Forestry Science, Beijing, 100097, China.
Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, China.
Mol Hortic. 2024 Feb 19;4(1):5. doi: 10.1186/s43897-024-00082-7.
N-acetylcytidine (acC) modification of mRNA has been shown to be present in plant RNAs, but its regulatory function in plant remains largely unexplored. In this study, we investigated the differentially expressed mRNAs, lncRNAs and acetylation modifications of mRNAs in tomato fruits from both genotypes. By comparing wild-type (AC) tomato and the ethylene receptor-mutant (Nr) tomato from mature green (MG) to six days after the breaker (Br6) stage, we identified differences in numerous key genes related to fruit ripening and observed the corresponding lncRNAs positively regulated the target genes expression. At the post-transcriptional level, the acetylation level decreased and increased in AC and Nr tomatoes from MG to Br6 stage, respectively. The integrated analysis of RNA-seq and acC-seq data revealed the potential positive role of acetylation modification in regulating gene expression. Furthermore, we found differential acetylation modifications of certain transcripts (ACO, ETR, ERF, PG, CesA, β-Gal, GAD, AMY, and SUS) in AC and Nr fruits which may explain the differences in ethylene production, fruit texture, and flavor during their ripening processes. The present study provides new insights into the molecular mechanisms by which acetylation modification differentially regulates the ripening process of wild-type and mutant tomato fruits deficient in ethylene signaling.
N-乙酰胞苷（acC）对mRNA的修饰已在植物RNA中被证实存在，但其在植物中的调控功能仍 largely未被探索。在本研究中，我们调查了两种基因型番茄果实中差异表达的mRNA、lncRNA以及mRNA的乙酰化修饰情况。通过比较野生型（AC）番茄和乙烯受体突变体（Nr）番茄从绿熟期（MG）到破色期后6天（Br6）的情况，我们鉴定出了许多与果实成熟相关的关键基因的差异，并观察到相应的lncRNA对靶基因表达有正向调控作用。在转录后水平，从MG到Br6阶段，AC番茄中的乙酰化水平降低，而Nr番茄中的乙酰化水平升高。RNA-seq和acC-seq数据的综合分析揭示了乙酰化修饰在调控基因表达方面的潜在正向作用。此外，我们发现AC和Nr果实中某些转录本（ACO、ETR、ERF、PG、CesA、β-Gal、GAD、AMY和SUS）存在差异乙酰化修饰，这可能解释了它们在成熟过程中乙烯产生、果实质地和风味方面的差异。本研究为乙酰化修饰差异调控野生型和缺乏乙烯信号的突变体番茄果实成熟过程的分子机制提供了新的见解。
