Bhat Susheel S, Paul Michel, Gregory Brian D
Department of Biology, University of Pennsylvania, Philadelphia, PA, USA.
RNA Biol. 2025 Dec;22(1):1-14. doi: 10.1080/15476286.2025.2515663. Epub 2025 Jun 8.
A plethora of new and exciting findings have dramatically widened the horizons of plant RNA-related research. Despite being identified decades ago, RNA modifications were largely ignored owing to the immense difficulty in studying them with traditional methods. We now know that these chemical additions to RNA nucleotides affect a myriad of plant biological processes ranging from plant growth and development to stress responses. The field of epitranscriptomics, the study of RNA modifications, has been dominated by mA in messenger RNAs (mRNAs), while modifications other than mA remained largely unstudied. A recent increase in studies investigating other RNA modifications and the development of novel tools has added to the evolving landscape of plant epitranscriptomic research. As this non-mA RNA modification research gathers pace, we use this review to provide a snapshot of the current state-of-the-art regarding these modifications with a focus on those occurring in functional non-coding RNAs as compared to mRNAs.
大量新奇且令人兴奋的发现极大地拓宽了植物RNA相关研究的视野。尽管RNA修饰在数十年前就已被发现，但由于用传统方法研究它们存在巨大困难，所以在很大程度上被忽视了。我们现在知道，这些对RNA核苷酸的化学添加影响着无数的植物生物学过程，从植物生长发育到应激反应。表观转录组学领域，即RNA修饰的研究，一直以信使RNA（mRNA）中的N6-甲基腺苷（m6A）为主导，而除m6A之外的修饰在很大程度上仍未得到研究。最近，对其他RNA修饰的研究不断增加，以及新型工具的开发，为植物表观转录组学研究不断变化的格局增添了内容。随着这项非m6A RNA修饰研究的加速推进，我们利用这篇综述提供关于这些修饰的当前最新情况的概述，重点关注与mRNA相比，在功能性非编码RNA中发生的那些修饰。
