Winkler M E
J Bacteriol. 1979 Sep;139(3):842-9. doi: 10.1128/jb.139.3.842-849.1979.
Ribonucleic acid (RNA) isolated by four distinct methods and from a variety of Salmonella typhimurium strains lacked intact 23S ribosomal RNA (rRNA). On sucrose gradients which minimize aggregation, the vast majority of S. typhimurium rRNA sedimented as a 16S peak with a 14S shoulder. RNA from this region of the gradient was resolved into three discrete bands by electrophoresis in formamide. Two very minor S. typhimurium RNA peaks were resolved at 21S and 10S on sucrose gradients, and each peak formed discrete bands in electrophoresis. It is concluded that if S. typhimurium does possess an intact 23S rRNA species, this species is extremely "labile." The absence of isolatable S. typhimurium 23S rRNA possibly reflected in vivo processing of the rRNA before isolation. Under certain conditions, S. typhimurium rRNA formed discrete aggregates which sedimented similarly to intact Escherichia coli 23S rRNA.
通过四种不同方法从多种鼠伤寒沙门氏菌菌株中分离得到的核糖核酸（RNA）缺乏完整的23S核糖体RNA（rRNA）。在能将聚集作用降至最低的蔗糖梯度中，绝大多数鼠伤寒沙门氏菌rRNA以16S峰和14S肩峰的形式沉降。梯度该区域的RNA通过在甲酰胺中电泳被分离成三条离散条带。在蔗糖梯度上，两个非常小的鼠伤寒沙门氏菌RNA峰在21S和10S处被分辨出来，并且每个峰在电泳中形成离散条带。得出的结论是，如果鼠伤寒沙门氏菌确实拥有完整的23S rRNA种类，那么该种类极其“不稳定”。无法分离到鼠伤寒沙门氏菌23S rRNA可能反映了在分离前rRNA在体内的加工过程。在某些条件下，鼠伤寒沙门氏菌rRNA形成离散聚集体，其沉降方式与完整的大肠杆菌23S rRNA相似。
