New alleles of , , and demonstrate that they are dispensable for stress-induced sleep in .

Sleep is ancient and genetically conserved across phylogeny. Neuropeptide signaling plays a fundamental role in the regulation of sleep for mammals, fish, and invertebrates like . Developmentally timed-sleep and stress-induced sleep of are controlled by distinct and overlapping neuropeptide pathways. The RPamide neuropeptides , , and , play antagonistic roles during the regulation of developmentally-timed sleep, however, their role in stress-induced sleep has not been explored. These genes are linked on the X chromosome, which has made genetic analyses challenging. Here we used CRISPR to generate new alleles of and , ; double mutants, and ; ; triple mutants. Confirming previous studies, we find that is required for developmentally-timed sleep, and show that is also required. However, all three genes are dispensable for stress-induced sleep.