Churgin Matthew A, Jung Sang-Kyu, Yu Chih-Chieh, Chen Xiangmei, Raizen David M, Fang-Yen Christopher
Department of Bioengineering, School of Engineering and Applied Sciences, University of Pennsylvania, Philadelphia, United States.
Department of Neurology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, United States.
Elife. 2017 May 31;6:e26652. doi: 10.7554/eLife.26652.
The roundworm is a mainstay of aging research due to its short lifespan and easily manipulable genetics. Current, widely used methods for long-term measurement of are limited by low throughput and the difficulty of performing longitudinal monitoring of aging phenotypes. Here we describe the WorMotel, a microfabricated device for long-term cultivation and automated longitudinal imaging of large numbers of confined to individual wells. Using the WorMotel, we find that short-lived and long-lived strains exhibit patterns of behavioral decline that do not temporally scale between individuals or populations, but rather resemble the shortest and longest lived individuals in a wild type population. We also find that behavioral trajectories of worms subject to oxidative stress resemble trajectories observed during aging. Our method is a powerful and scalable tool for analysis of behavior and aging.
蛔虫因其寿命短和易于操作的遗传学特性，成为衰老研究的主要对象。目前广泛使用的长期测量方法存在通量低以及难以对衰老表型进行纵向监测的问题。在此，我们介绍一种微制造装置WorMotel，它可用于对大量置于单个孔中的蛔虫进行长期培养和自动纵向成像。使用WorMotel，我们发现短寿命和长寿命品系表现出行为衰退模式，这种模式在个体或群体之间不存在时间上的比例关系，而是类似于野生型群体中最短寿和最长寿的个体。我们还发现，遭受氧化应激的蛔虫的行为轨迹类似于衰老过程中观察到的轨迹。我们的方法是分析蛔虫行为和衰老的强大且可扩展的工具。
